Medullins A-H, Sesquiterpenes from Perenniporia medulla-panis, and Their Cellular Signaling Mechanism in HDF Cells

IF 3.3 2区生物学 Q2 CHEMISTRY, MEDICINAL Journal of Natural Products Pub Date : 2024-09-18 DOI:10.1021/acs.jnatprod.4c00436

Dae-Won Ki, Ji-Yul Kim, Young-Hee Lee, Dae-Cheol Choi, Ho-Keun Yi, In-Kyoung Lee, Bong-Sik Yun

引用次数: 0

Abstract

A chemical investigation of an ethyl acetate-soluble layer in the culture broth of Perenniporia medulla-panis resulted in the isolation of eight novel sesquiterpenes conjugated Gly (1), l-Val (2), l-Ala (3), l-Tyr (4), l-Thr (5), l-Ile (6), l-Leu (7), and l-Phe (8). Elucidation of their structures was performed through comprehensive spectroscopic analysis. The absolute configuration of the sesquiterpene skeleton was ascertained using modified Mosher’s methods. The configurations of the amino acid units in compounds 2–8 were identified through acid hydrolysis followed by LC-MS analysis employing Marfey’s method. Compounds 1–3 and 5–8 showed significant regulating effect on MAP kinase activity (p-ERK and p-JNK) in human diploid fibroblast (HDF) cells.

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髓苷 A-H（来自秘鲁髓苷的倍半萜）及其在 HDF 细胞中的细胞信号机制

通过对髓质长春花（Perenniporia medulla-panis）培养液中的乙酸乙酯可溶层进行化学研究，分离出了八种新的倍半萜类化合物，它们分别与 Gly (1)、l-Val (2)、l-Ala (3)、l-Tyr (4)、l-Thr (5)、l-Ile (6)、l-Leu (7) 和 l-Phe (8) 共轭。通过全面的光谱分析阐明了它们的结构。倍半萜骨架的绝对构型是用改进的莫舍方法确定的。化合物 2-8 中氨基酸单元的构型是通过酸水解，然后采用 Marfey 方法进行 LC-MS 分析确定的。化合物 1-3 和 5-8 对人二倍体成纤维细胞（HDF）中的 MAP 激酶活性（p-ERK 和 p-JNK）具有显著的调节作用。

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.