Rosa Nicole Freiberger, Cynthia Alicia Marcela López, María Belén Palma, Cintia Cevallos, Franco Agustin Sviercz, Patricio Jarmoluk, Marcela Nilda García, Jorge Quarleri, M. Victoria Delpino
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引用次数: 0
Abstract
Bone loss is a prevalent characteristic among people with HIV (PWH). We focused on mesenchymal stem cells (MSCs) and osteoblasts, examining their susceptibility to different HIV strains (R5- and X4-tropic) and the subsequent effects on bone tissue homeostasis. Our findings suggest that MSCs and osteoblasts are susceptible to R5- and X4-tropic HIV but do not support productive HIV replication. HIV exposure during the osteoblast differentiation process revealed that the virus could not alter mineral and organic matrix deposition. However, the reduction in runt-related transcription factor 2 (RUNX2) transcription, the increase in the transcription of nuclear receptor activator ligand kappa B (RANKL), and the augmentation of vitronectin deposition strongly suggested that X4- and R5-HIV could affect bone homeostasis. This study highlights the HIV ability to alter MSCs’ differentiation into osteoblasts, critical for maintaining bone and adipose tissue homeostasis and function.
骨质流失是艾滋病病毒感染者(PWH)的一个普遍特征。我们重点研究了间充质干细胞(MSCs)和成骨细胞,考察了它们对不同 HIV 株系(R5-和 X4-tropic)的易感性以及随后对骨组织稳态的影响。我们的研究结果表明,间充质干细胞和成骨细胞对 R5 和 X4-Tropic HIV 易感,但不支持 HIV 的生产性复制。在成骨细胞分化过程中暴露于艾滋病毒的情况表明,病毒不会改变矿物质和有机基质的沉积。然而,与RUNT相关的转录因子2(RUNX2)转录的减少、核受体激活配体卡巴B(RANKL)转录的增加以及玻璃连蛋白沉积的增加都有力地表明,X4和R5-HIV可影响骨平衡。这项研究强调了艾滋病毒改变间充质干细胞向成骨细胞分化的能力,而成骨细胞对维持骨骼和脂肪组织的稳态和功能至关重要。
期刊介绍:
Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.