Xiuli Wang, Leyi He, Yaoxia Li, Jia Guo, Changchun Wang
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引用次数: 0
Abstract
High-sensitivity detection of biomarkers in biological samples is crucial for the early diagnosis and treatment of diseases. In this paper, a versatile and flexible immobilization technique based on the specific affinity interaction between boronic acid and cis-diol groups of antibodies was developed for biomarker detection. As a model, the boronic acid-modified immunomagnetic beads were used for facile and quick immobilization of the alpha-fetoprotein (AFP) antibody due to the specific affinity interactions. Based on this new class of immunomagnetic beads, the chemiluminescence immunosensor could efficiently detect the biomarker of AFP. Under optimal conditions, the limit of detection (LOD) is as low as 8 fM (S/N = 3), showcasing superior sensitivity and detection specificity for AFP. Subsequently, the system was successfully applied to the detection of AFP in fetal bovine serum samples, and the average recovery rate is greater than 95%. Its performance surpassed that of commercial immunomagnetic beads, showcasing the potential application of this new strategy for bioanalysis and clinical diagnosis.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices