In Vitro Anti-Shigella, Antioxidant Activities, and Oral Acute Toxicity of Organics Extracts from the Root Bark of Detarium microcarpum Guill. and Perr.

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Journal of Tropical Medicine Pub Date : 2024-09-06 DOI:10.1155/2024/1330063
Hama Hamadou Habibou,Mahamane Idi Issa Abdoulahi,Ikhiri Khalid
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Abstract

Detarium microcarpum (Fabaceae) is a medicinal plant from the traditional pharmacopeia of Niger used against gastrointestinal disorders and dysentery. This study was designed to assess the in vitro anti-shigella, antioxidant activities, and oral acute toxicity of extract root barks of Detarium microcarpum. The crude extracts were prepared by maceration using methanol, ethanol, dichloromethane, water-ethanol (30/70 v/v), and methanol-dichloromethane (1/1 v/v). The anti-shigella activity was performed using the microdilution method coupled with the resazurin-based assay. The antioxidant activity was evaluated by the DPPH· (2, 2-diphényl-1-picrylhydrazyl), ABTS 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), and H2O2 assays. The oral acute toxicity was assessed following the Organization for Economic Cooperation and Development (OECD) guidelines. The extracts displayed activity against the Shigella boydii with Minimum Inhibitory Concentrations (MICs) from 500 to 1000 μg/mL. The methanolic crude extract of D. microcarpum shows good antioxidant activity with the radicals DPPH· and ABTS with inhibitory concentration 50 (IC50) at 228 and 191 µg/mL, respectively. The lethal dose 50 (LD50) of extract was up to 2000 mg/kg of body weight, and no signs of toxicity were observed. These findings supported the use of Detarium microcarpum in the traditional treatment of gastrointestinal disorders.
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Detarium microcarpum Guill.
Detarium microcarpum(豆科)是尼日尔传统药典中的一种药用植物,用于治疗肠胃疾病和痢疾。本研究旨在评估Detarium microcarpum根皮提取物的体外抗志贺氏杆菌、抗氧化活性和口服急性毒性。粗提取物的制备采用甲醇、乙醇、二氯甲烷、水-乙醇(30/70 v/v)和甲醇-二氯甲烷(1/1 v/v)浸渍法。抗志贺氏菌活性采用微量稀释法和基于利马唑啉的测定法。抗氧化活性通过 DPPH-(2, 2-二苯基-1-苦基肼)、ABTS 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) 和 H2O2 试验进行评估。根据经济合作与发展组织(OECD)的指导方针,对口服急性毒性进行了评估。提取物对男童志贺氏菌具有活性,最低抑制浓度(MICs)为 500 至 1000 μg/mL。D. microcarpum 的甲醇粗提取物对 DPPH- 和 ABTS 自由基具有良好的抗氧化活性,其抑制浓度 50 (IC50) 分别为 228 和 191 μg/mL。萃取物的致死剂量50(LD50)为2000毫克/千克体重,未观察到任何毒性迹象。这些研究结果支持使用Detarium microcarpum来治疗传统的胃肠道疾病。
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来源期刊
Journal of Tropical Medicine
Journal of Tropical Medicine Immunology and Microbiology-Parasitology
CiteScore
3.90
自引率
4.50%
发文量
0
审稿时长
14 weeks
期刊介绍: Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
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