Wei Du, Mingxuan Zhou, Guancheng Wang, Mingze Lu, Xiao Wang, Zhirui Guo, Ming Ma, Yu Zhang
{"title":"HPRR-Based Diatomic Catalyst Electrochemistry Biosensor for Detecting Cancer-Related Extracellular Vesicles","authors":"Wei Du, Mingxuan Zhou, Guancheng Wang, Mingze Lu, Xiao Wang, Zhirui Guo, Ming Ma, Yu Zhang","doi":"10.1039/d4ay01573h","DOIUrl":null,"url":null,"abstract":"Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis due to their rich content of tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR) based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst catalytic HPRR current amplification strategies for specific identification and highly sensitive detection. Anti-CXCR4 antibody was immobilized on Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bounded to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 107 particles mL-1, with a detection limit of 117 particles mL-1, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01573h","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis due to their rich content of tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR) based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst catalytic HPRR current amplification strategies for specific identification and highly sensitive detection. Anti-CXCR4 antibody was immobilized on Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bounded to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 107 particles mL-1, with a detection limit of 117 particles mL-1, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.