A novel laccase for alkaline medium temperature environments in the textile industry

IF 3.2 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Biotechnology Journal Pub Date : 2024-09-19 DOI:10.1002/biot.202400383

Kaifeng Xu, Ying Huo, Shiming Tang, Shuangyan Han, Ying Lin, Suiping Zheng

引用次数: 0

Abstract

Laccases are extensively used in the textile industry due to their ability to decolorize dyes, modify fabric surfaces, and bleach textiles. Identifying a laccase with both high thermal stability and alkali tolerance suitable for textile applications presents a significant challenge. A novel alkaline laccase, LacCT, was discovered from Caldalkalibacillus thermarum and successfully expressed it in Escherichia coli. LacCT displayed optimal activity at 65°C and maintained high stability across a pH range of 6.0–10.0, with an optimal pH of 7.5. Through rational design, the thermal stability of the best variant, G190P/Q254Y/G336M/D510F (LacCT-11), was significantly enhanced, resulting in a half-life of 63.2 min at 60°C – 1.8 times longer than that of the wild type. This research introduces a promising new laccase with considerable potential for decolorizing textile wastewater and improving the ramie degumming process.

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适用于纺织业碱性中温环境的新型漆酶

由于漆酶具有染料脱色、织物表面改性和纺织品漂白的能力，因此被广泛应用于纺织业。鉴定一种具有高热稳定性和耐碱性、适合纺织品应用的漆酶是一项重大挑战。研究人员从热钙碱性杆菌（Caldalkalibacillus thermarum）中发现了一种新型碱性漆酶 LacCT，并将其成功地表达在大肠杆菌中。LacCT 在 65°C 时显示出最佳活性，并在 6.0-10.0 的 pH 值范围内保持高稳定性，最佳 pH 值为 7.5。通过合理设计，最佳变体 G190P/Q254Y/G336M/D510F （LacCT-11）的热稳定性显著提高，在 60°C 时的半衰期为 63.2 分钟，是野生型的 1.8 倍。这项研究为纺织废水脱色和改善苎麻脱胶工艺引入了一种前景广阔的新型漆酶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.