Transcriptional Activity of Genes Regulating T-Helper Differentiation in the Accidentally Exposed Population of the Southern Urals

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Doklady Biochemistry and Biophysics Pub Date : 2024-09-16 DOI:10.1134/s1607672924701114

V. S. Nikiforov, A. I. Kotikova, E. A. Blinova, A. V. Akleyev

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Abstract

The objective of this work was to study the expression of the TBX21, RORC, GATA3, NFKB1, MAPK8, and STAT3 genes responsible for the regulation of the differentiation of various T-helper subpopulations in individuals chronically exposed to radiation. The object of the study was peripheral blood cells obtained from 120 persons chronically exposed to radiation in a wide range of doses on the Techa River. The mean cumulative absorbed dose to red bone marrow in the examined exposed individuals was 742.7 ± 78.6 mGy (dose range, 73.5–3516.1 mGy); in the comparison group, 17.4 ± 2.2 mGy (dose range, 0.0–55.5 mGy). The subpopulation composition of T-helpers (Th1, Th2, and Th17) was analyzed by flow cytofluorometry. The relative mRNA content of the TBX21, RORC, GATA3, NFKB1, MAPK8, and STAT3 genes was estimated by real-time PCR. The study made it possible to note a decrease in the relative number of T-helpers 2 in the populations of T-helpers of the central memory in the group of chronically exposed persons compared to the comparison group. In the population of T-helpers of the central memory, a statistically significant increase in the relative number of T-helpers 1 was shown, depending on the accumulated absorbed dose to red bone marrow. No changes in mRNA expression of the studied genes were observed. The analysis of the correlation between the expression of GATA3, MAPK8, STAT3, RORC, and TBX21 mRNA and the relative number of cells in subpopulations of T-helper types 1, 2, and 17 in the examined people did not reveal statistically significant patterns.

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南乌拉尔地区意外暴露人群中调节 T 细胞分化基因的转录活性

摘要这项工作的目的是研究长期暴露于辐射的人体内负责调节各种 T 辅助亚群分化的 TBX21、RORC、GATA3、NFKB1、MAPK8 和 STAT3 基因的表达情况。研究对象是 120 名长期暴露于特查河大剂量辐射的人的外周血细胞。受检者红骨髓的平均累积吸收剂量为 742.7 ± 78.6 mGy（剂量范围为 73.5-3516.1 mGy）；对比组为 17.4 ± 2.2 mGy（剂量范围为 0.0-55.5 mGy）。流式细胞荧光测定法分析了T辅助细胞（Th1、Th2和Th17）的亚群组成。实时 PCR 评估了 TBX21、RORC、GATA3、NFKB1、MAPK8 和 STAT3 基因的相对 mRNA 含量。研究结果表明，与对比组相比，长期暴露者组的中枢记忆 T 辅助细胞群中 T 辅助细胞 2 的相对数量有所减少。在中枢记忆的 T 辅助细胞群中，T 辅助细胞 1 的相对数量出现了统计学意义上的显著增加，这取决于红骨髓的累积吸收剂量。所研究基因的 mRNA 表达没有变化。对受检者中 GATA3、MAPK8、STAT3、RORC 和 TBX21 mRNA 的表达与 1、2 和 17 型 T 辅助细胞亚群中细胞相对数量之间的相关性进行分析，并未发现有统计学意义的模式。

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.