Arabidopsis F‐box proteins D5BF1 and D5BF2 negatively regulate Agrobacterium‐mediated transformation and tumorigenesis

IF 4.8 1区农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-09-13 DOI:10.1111/mpp.70006

Qin Hu, Xueying Li, Weijie Xi, Junjie Xu, Chao Xu, Israel Ausin, Yafei Wang

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Abstract

The pathogen Agrobacterium tumefaciens is known for causing crown gall tumours in plants. However, it has also been harnessed as a valuable tool for plant genetic transformation. Apart from the T‐DNA, Agrobacterium also delivers at least five virulence proteins into the host plant cells, which are required for an efficient infection. One of these virulence proteins is VirD5. F‐box proteins, encoded in the host plant genome or the Ti plasmid, and the ubiquitin/26S proteasome system (UPS) also play an important role in facilitating Agrobacterium infection. Our study identified two Arabidopsis F‐box proteins, D5BF1 and D5BF2, that bind VirD5 and facilitate its degradation via the UPS. Additionally, we found that Agrobacterium partially suppresses the expression of D5BF1 and D5BF2. Lastly, stable transformation and tumorigenesis efficiency assays revealed that D5BF1 and D5BF2 negatively regulate the Agrobacterium infection process, showing that the plant F‐box proteins and UPS play a role in defending against Agrobacterium infection.

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拟南芥 F-box 蛋白 D5BF1 和 D5BF2 负向调节农杆菌介导的转化和肿瘤发生

众所周知，病原体农杆菌（Agrobacterium tumefaciens）会导致植物冠瘿瘤。不过，它也被用作植物基因转化的重要工具。除了 T-DNA 外，农杆菌还向宿主植物细胞提供至少五种有效感染所需的毒力蛋白。VirD5 就是其中一种毒力蛋白。宿主植物基因组或 Ti 质粒中编码的 F-box 蛋白和泛素/26S 蛋白酶体系统（UPS）也在促进农杆菌感染方面发挥着重要作用。我们的研究发现了两个拟南芥 F-box 蛋白 D5BF1 和 D5BF2，它们能结合 VirD5 并通过 UPS 促进其降解。此外，我们还发现农杆菌会部分抑制 D5BF1 和 D5BF2 的表达。最后，稳定转化和肿瘤发生效率试验显示，D5BF1 和 D5BF2 对农杆菌感染过程有负向调节作用，这表明植物 F-box 蛋白和 UPS 在抵御农杆菌感染方面发挥了作用。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.