{"title":"EMS-induced missense mutation in TaCHLI-7D affects leaf color and yield-related traits in wheat","authors":"Zixu Wang, Huiyuan Xu, Faxiang Wang, Lingling Sun, Xiangrui Meng, Zhuochun Li, Chang Xie, Huijiao Jiang, Guangshuo Ding, Xinrong Hu, Yuhang Gao, Ran Qin, Chunhua Zhao, Han Sun, Fa Cui, Yongzhen Wu","doi":"10.1007/s00122-024-04740-8","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Key message</h3><p>Mutations in<i> TaCHLI</i> impact chlorophyll levels and yield-related traits in wheat.\nNatural variations in<i> TaCHLI-7A/B</i> influence plant productivity, offering potential for molecular\nbreeding.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Chlorophyll is essential for plant growth and productivity. The CHLI subunit of the magnesium chelatase protein plays a key role inserting magnesium into protoporphyrin IX during chlorophyll biosynthesis. Here, we identify a novel wheat mutant <i>chlorophyll</i> (<i>chl</i>) that exhibits yellow-green leaves, reduced chlorophyll levels, and increased carotenoid content, leading to an overall decline in yield-related traits. Map-based cloning reveals that the <i>chl</i> phenotype is caused by a point mutation (Asp186Asn) in the <i>TaCHLI-7D</i> gene, which encodes subunit I of magnesium chelatase. Furthermore, the three <i>TaCHLI</i> mutants: <i>chl-7b-1</i> (Pro82Ser)<i>, chl-7b-2</i> (Ala291Thr), and <i>chl-7d-1</i> (Gly357Glu), also showed significant reductions in chlorophyll content and yield-related traits. However, <i>TaCHLI-7D</i> overexpression in rice significantly decreased thousand kernel weight, yield per plant, and germination. Additionally, natural variations in <i>TaCHLI-7A/B</i> are significantly associated with flag leaf, spike exsertion length, and yield per plant. Notably, the favorable haplotype, <i>TaCHLI-7B-HapII</i>, which displayed higher thousand kernel weight and yield per plant, is positively selected in wheat breeding. Our study provides insights on the regulatory molecular mechanisms underpinning leaf color and chlorophyll biosynthesis, and highlights <i>TaCHLI</i> functions, which provide useful molecular markers and genetic resources for wheat breeding.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"31 1","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theoretical and Applied Genetics","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s00122-024-04740-8","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Key message
Mutations in TaCHLI impact chlorophyll levels and yield-related traits in wheat.
Natural variations in TaCHLI-7A/B influence plant productivity, offering potential for molecular
breeding.
Abstract
Chlorophyll is essential for plant growth and productivity. The CHLI subunit of the magnesium chelatase protein plays a key role inserting magnesium into protoporphyrin IX during chlorophyll biosynthesis. Here, we identify a novel wheat mutant chlorophyll (chl) that exhibits yellow-green leaves, reduced chlorophyll levels, and increased carotenoid content, leading to an overall decline in yield-related traits. Map-based cloning reveals that the chl phenotype is caused by a point mutation (Asp186Asn) in the TaCHLI-7D gene, which encodes subunit I of magnesium chelatase. Furthermore, the three TaCHLI mutants: chl-7b-1 (Pro82Ser), chl-7b-2 (Ala291Thr), and chl-7d-1 (Gly357Glu), also showed significant reductions in chlorophyll content and yield-related traits. However, TaCHLI-7D overexpression in rice significantly decreased thousand kernel weight, yield per plant, and germination. Additionally, natural variations in TaCHLI-7A/B are significantly associated with flag leaf, spike exsertion length, and yield per plant. Notably, the favorable haplotype, TaCHLI-7B-HapII, which displayed higher thousand kernel weight and yield per plant, is positively selected in wheat breeding. Our study provides insights on the regulatory molecular mechanisms underpinning leaf color and chlorophyll biosynthesis, and highlights TaCHLI functions, which provide useful molecular markers and genetic resources for wheat breeding.
期刊介绍:
Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.