Exploring Trypanosoma cruzi transmission dynamics in an acute Chagas disease outbreak using next-generation sequencing

IF 3 2区医学 Q1 PARASITOLOGY Parasites & Vectors Pub Date : 2024-09-18 DOI:10.1186/s13071-024-06445-9

Lissa Cruz-Saavedra, Carlos Ospina, Stivenn A. Gutiérrez, Jeiczon Jaimes-Dueñez, Omar Cantillo-Barraza, Carolina Hernández, Francisco Álvarez, María Blanco, Bernardo Leal, Lida Martínez, Manuel Medina, Mabel Medina, Silvia Valdivieso, Lauren Natalia Ramirez Celis, Luz H. Patiño, Juan David Ramírez

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Abstract

Chagas disease (CD), caused by Trypanosoma cruzi, poses a major global public health challenge. Although vector-borne transmission is the primary mode of infection, oral transmission is increasingly concerning. This study utilized long-amplicon-based sequencing (long-ABS), focusing on the 18S rRNA gene, to explore T. cruzi’s genetic diversity and transmission dynamics during an acute CD outbreak in Colombia, an area without domestic infestation. Analyzing samples from five patients and five T. cruzi-positive marsupial samples, we identified coinfections between T. cruzi and Trypanosoma rangeli, mixed T. cruzi DTUs, suggesting possible links between human and marsupial T. cruzi infections. Coexistence of TcI, TcIV and T. rangeli suggests marsupial secretions as the possible source of T. cruzi transmission. Our investigation revealed diversity loss in DTUs TcIV and T. rangeli in humans after infection and in marsupial samples after culture. These findings provide significant insights into T. cruzi dynamics, crucial for implementing control and prevention strategies.

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利用下一代测序技术探索南美锥虫病急性爆发期的传播动态

由克鲁兹锥虫引起的恰加斯病（CD）是全球公共卫生面临的一大挑战。虽然病媒传播是主要的感染模式，但口腔传播也越来越令人担忧。这项研究利用基于长扩增子的测序（long-ABS）技术，以18S rRNA基因为重点，探讨了在哥伦比亚（一个没有国内侵扰的地区）爆发急性CD疫情期间，克鲁兹锥虫的遗传多样性和传播动态。通过分析五例患者样本和五例T. cruzi阳性有袋动物样本，我们发现了T. cruzi和Trypanosoma rangeli、混合T. cruzi DTUs之间的共感染，这表明人类和有袋动物T. cruzi感染之间可能存在联系。TcI、TcIV和T. rangeli的共存表明有袋类动物的分泌物可能是T. cruzi的传播源。我们的调查发现，在人类感染后和有袋动物样本培养后，DTUs TcIV 和 T. rangeli 的多样性丧失。这些发现为我们提供了有关 T. cruzi 动态的重要信息，对于实施控制和预防策略至关重要。

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来源期刊

Parasites & Vectors 医学-寄生虫学

CiteScore

6.30

自引率

9.40%

发文量

433

审稿时长

1.4 months

期刊介绍： Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.