Morphological and molecular identification of Euclinostomum heterostomum (Trematoda: Clinostomidae) from spotted snakehead Channa punctata in Bangladesh

IF 1 4区医学 Q4 PARASITOLOGY Systematic Parasitology Pub Date : 2024-09-19 DOI:10.1007/s11230-024-10175-y

Sayed Mashequl Bari, Aktia Amina, Zubyda Mushtari Nadia, Raf Ana Rabbi Shawon, Md. Matiur Rahman, Md. Abdul Masum, Kazi Ahsan Habib

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Abstract

Parasites belonging to the trematode family Clinostomidae have a global distribution. Freshwater fish in Bangladesh frequently serve as hosts for different clinostomid species. During the metacercaria stage, the digenetic trematode Euclinostomum heterostomum exhibits a specific preference for Channidae species, where it undergoes encystment within the liver, kidneys, and muscles of these hosts. The study aimed to identify E. heterostomum in Channa punctata through combined morphological and molecular analysis. Light and scanning electron microscopy were conducted to investigate the morphological characteristics of the species. The histological examination of the infected liver tissue revealed the presence of encysted flukes, inflammatory leukocyte infiltrates, and degeneration and loosening of hepatic tissue. In molecular analysis, the generated internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA and ITS2 regions sequence (GenBank accession no: OR591452) of 830 bp showed 100% identity with Euclinostomum heterostomum identified in India (MT785786). The phylogenetic reconstruction provided substantial evidence of genetic similarities (0–1% genetic distance) among different isolates of the genus Euclinostomum, suggesting that there is a shared heritage.

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从孟加拉国斑点乌鳢中发现的 Euclinostomum heterostomum（吸虫纲：Clinostomidae）的形态学和分子鉴定

属于震颤虫科的寄生虫在全球都有分布。孟加拉国的淡水鱼经常是不同栉水母科鱼类的宿主。在元蛔虫阶段，异蛔虫（Euclinostomum heterostomum）表现出对鳢科鱼类的特殊偏好，它会在这些宿主的肝脏、肾脏和肌肉中发生包囊。本研究旨在通过形态学和分子分析来鉴定点鳢中的异刺鳗。研究人员利用光学显微镜和扫描电子显微镜对该物种的形态特征进行了研究。受感染肝组织的组织学检查显示存在包囊吸虫、炎性白细胞浸润、肝组织变性和松弛。在分子分析中，生成的内部转录间隔 1（ITS1）、5.8S 核糖体 DNA 和 ITS2 区域序列（GenBank 编号：OR591452）共 830 bp，与在印度发现的 Euclinostomum heterostomum（MT785786）具有 100%的相同性。系统发育重建提供了 Euclinostomum 属不同分离株之间遗传相似性（遗传距离为 0-1%）的大量证据，表明存在共同的遗产。

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来源期刊

Systematic Parasitology 医学-寄生虫学

CiteScore

2.20

自引率

23.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Systematic Parasitology publishes papers on the systematics, taxonomy and nomenclature of the following groups: Nematoda (including plant-parasitic), Monogenea, Digenea, Cestoda, Acanthocephala, Aspidogastrea, Cestodaria, Arthropoda (parasitic copepods, hymenopterans, mites, ticks, etc.), Protozoa (parasitic groups), and parasitic genera in other groups, such as Mollusca, Turbelleria, etc. Systematic Parasitology publishes fully illustrated research papers, brief communications, and fully illustrated major revisions. In order to maintain high standards, all contributors describing new taxa are asked to state clearly where the holotype is deposited and to make paratypes available for examination by the referees. It is recognized that, in some cases, this may cause problems for the authors, but it is hoped that by adhering to this rule authors may be protected against rapid synonymy of their taxa, and the types will be preserved for posterity.