Exploring the molecular biology of zinc-doped hydroxyapatite nanocomposites as fillers for dental materials: a self-defensive approach targeting bacterial DNA

IF 1.8 4区 材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Materials Research Express Pub Date : 2024-09-12 DOI:10.1088/2053-1591/ad7785
Muhammad Hamza, Qudsia Kanwal, Zahid Ali, Seema Zargar, Atekah Hazzaa Alshammari, Tanveer A Wani, Muhammad Rizwan, Kanwal Rohm and Muhammad Asim Mushtaq
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Abstract

Infections during or after the denture filling pose a serious threat to recovery later, and antibiotics are scanty for quick healing. The antibacterial potential of Zn has convinced us to fabricate its composites with hydroxyapatite (HA), which has distinct biological properties. The main aim of the study is to evaluate the antibacterial potential of Zn-doped HA and investigate its molecular interaction with bacterial strains to enhance its defensive ability against bacterial attack. This study investigates the synthesis of zinc-doped hydroxyapatite nano-powder (Zn-HA) as coating and filling materials for dental applications. The co-precipitation technique was used in this regard to obtain the purest form of nanoparticles. The as-prepared Zn-HA samples were characterized by FTIR spectroscopy to assess the interaction of functional groups between components, x-ray diffraction (XRD) to determine percentage crystallinity, SEM to explore the surface morphology of composites, and EDX to confirm the incorporation of Zn in the apatite structure. The crystalline size of Zn-HA decreases from 99 nm to 40 nm with an increase in the doping of Zncl2 from 0 to 2.5 g. With increasing the concentration of Zn doping, the effectiveness of antibacterial potential was increased. Due to an increased in the concentration of doped Zn, the Zn ions effectively rupture the bacterial membrane and destroy its DNA. Meanwhile, it shows the highest antibacterial activity against Pseudomonas (20.2 ± 0.02), Klebsiella (25.8 ± 0.05), Bacillus (18.3 ± 0.09), S. aureus (24 ± 0.03), and E. coli (19.3 ± 0.09) with Zn/HA5. It showed that by increasing the concentration of Zinc ions in hydroxyapatite, the antibacterial potential increased. This increased concentration of zinc ions in HA enhances its-defensive ability against the attack of various bacterial strains, this makes it a potential material and enhances its efficacy in dental applications like coating, filling material, or dental restoratives, in inhibiting the growth of bacterial colonies and biofilm formation in the oral cavity. In this way, Zn/HA as an efficient dental composite as a restorative material with enhanced antibacterial potential may help to mitigate the risk of dental infections, promote dental as well as oral health, and overall improve the life of dentine.
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探索作为牙科材料填充物的掺锌羟基磷灰石纳米复合材料的分子生物学:针对细菌 DNA 的自我防御方法
在义齿充填过程中或充填后发生感染会严重威胁后期的恢复,而抗生素又很难快速治愈。Zn 的抗菌潜力促使我们将其与具有独特生物特性的羟基磷灰石(HA)制成复合材料。本研究的主要目的是评估掺锌 HA 的抗菌潜力,并研究其与细菌菌株的分子相互作用,以增强其抵御细菌侵袭的能力。本研究探讨了掺锌羟基磷灰石纳米粉体(Zn-HA)作为牙科应用涂层和填充材料的合成。为了获得最纯净的纳米颗粒,本研究采用了共沉淀技术。制备的 Zn-HA 样品通过傅立叶变换红外光谱(FTIR spectroscopy)、X 射线衍射(XRD)和扫描电子显微镜(SEM)进行了表征,前者用于评估成分间官能团的相互作用,后者用于确定结晶度百分比,前者用于探究复合材料的表面形态,后者用于确认磷灰石结构中的锌含量。随着 Zncl2 掺杂浓度从 0 g 增加到 2.5 g,Zn-HA 的结晶尺寸从 99 nm 减小到 40 nm。由于掺杂 Zn 的浓度增加,Zn 离子能有效地破坏细菌膜并破坏其 DNA。同时,Zn/HA5 对假单胞菌(20.2 ± 0.02)、克雷伯氏菌(25.8 ± 0.05)、芽孢杆菌(18.3 ± 0.09)、金黄色葡萄球菌(24 ± 0.03)和大肠杆菌(19.3 ± 0.09)的抗菌活性最高。这表明,随着羟基磷灰石中锌离子浓度的增加,抗菌潜力也随之增加。羟基磷灰石中锌离子浓度的增加增强了其抵御各种细菌菌株攻击的能力,这使其成为一种潜在的材料,并提高了其在牙科应用(如涂层、填充材料或牙科修复体)中抑制口腔中细菌菌落生长和生物膜形成的功效。因此,Zn/HA 作为一种高效的牙科复合材料,作为一种具有更强抗菌潜力的修复材料,可能有助于降低牙科感染的风险,促进牙齿和口腔健康,并从整体上改善牙本质的寿命。
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来源期刊
Materials Research Express
Materials Research Express MATERIALS SCIENCE, MULTIDISCIPLINARY-
CiteScore
4.50
自引率
4.30%
发文量
640
审稿时长
12 weeks
期刊介绍: A broad, rapid peer-review journal publishing new experimental and theoretical research on the design, fabrication, properties and applications of all classes of materials.
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