The Protective Effect of Adipose Derived Stromal Vascular Fraction on Ovarian Function in Rats with Cyclophosphamide-Induced Ovarian Damage.

IF 2 4区 医学 Q2 OBSTETRICS & GYNECOLOGY Gynecologic and Obstetric Investigation Pub Date : 2024-09-12 DOI:10.1159/000541049
Sabri Berkem Okten,Pinar Ozcan,Olgu Enis Tok,Belgin Devranoglu,Caglar Cetin,Fatma Basak Tanoglu,Cem Ficicioglu
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Abstract

OBJECTIVE To investigate if adipose derived stromal vascular fraction (SVF) treatment has any protective effect on ovarian function in rats with cyclophosphamide (CP) induced ovarian damage. DESIGN Experimental animal study. Participants/ Materials, Setting, Methods: 25 mature cycling Wistar-Albino rats were randomized into four groups (n=5 per group). Rats in Group 1 and 2 received single dose of intraperitoneal (i.p.) 1 mL/kg sodium chloride 0.9% (NaCl). Group 3 and 4 received single dose of 75 mg/kg i.p. CP. On seventh day, SVF was prepared from adipose tissues of 5 additional rats and Group 1 and 3 received 0.9% NaCl i.p. injections while Group 2 and 4 received 0.2 mL i.p. injections of SVF. On day 21 all rats were euthanized, and serum anti-mullerian hormone (AMH) levels, primordial, primary, secondary, antral, and atretic follicle counts, AMH positive staining follicle counts along with AMH staining intensity of the follicles were evaluated. RESULTS Among two CP induced ovarian damaged groups, SVF treated group showed significantly higher secondary and antral follicle and lower atretic follicle counts, significantly higher mean serum AMH levels, AMH positive antral follicle count and higher intensity of AMH positive follicle scores for primary, secondary and antral follicles when compared to untreated group. Moreover, Group 1 showed no significant difference for all parameters except antral follicle count and AMH positive staining intensity scores for antral follicles when compared to Group 4. LIMITATIONS This study was conducted on experimental rat model. CONCLUSION Our study demonstrated a significant protective effect of SVF against Cp-induced ovarian damage which reveals the apparent need for further investigation of its precise mechanisms of action as it may provide a new treatment approach for women with premature ovarian failure.
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脂肪衍生基质血管组分对环磷酰胺诱发卵巢损伤大鼠卵巢功能的保护作用
设计实验性动物研究。参与者/材料、环境和方法:25 只成熟的循环 Wistar-Albino 大鼠被随机分为四组(每组 5 只)。第 1 组和第 2 组大鼠接受单剂量腹腔注射(i.p. )1 mL/kg 0.9% 氯化钠(NaCl)。第 3 组和第 4 组接受单剂量 75 毫克/千克氯化石蜡腹腔注射。第七天,从另外 5 只大鼠的脂肪组织中制备 SVF,第 1 组和第 3 组接受 0.9% 氯化钠静脉注射,第 2 组和第 4 组接受 0.2 mL SVF 静脉注射。第 21 天,对所有大鼠实施安乐死,并评估血清抗苗勒氏管激素(AMH)水平、原始卵泡、初级卵泡、次级卵泡、前区卵泡和闭锁卵泡计数、AMH 阳性染色卵泡计数以及卵泡的 AMH 染色强度。结果在两组 CP 诱导的卵巢损伤中,与未治疗组相比,SVF 治疗组的次级卵泡和喙卵泡数量明显增加,闭锁卵泡数量减少,平均血清 AMH 水平、AMH 阳性喙卵泡数量明显增加,初级卵泡、次级卵泡和喙卵泡的 AMH 阳性卵泡评分强度增加。此外,与第 4 组相比,第 1 组除窦前卵泡计数和窦前卵泡 AMH 阳性染色强度评分外,其他参数均无明显差异。结论我们的研究表明,SVF 对 Cp 引起的卵巢损伤有明显的保护作用,这表明显然需要进一步研究其确切的作用机制,因为它可能为卵巢早衰妇女提供一种新的治疗方法。
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来源期刊
CiteScore
4.20
自引率
4.80%
发文量
44
审稿时长
6-12 weeks
期刊介绍: This journal covers the most active and promising areas of current research in gynecology and obstetrics. Invited, well-referenced reviews by noted experts keep readers in touch with the general framework and direction of international study. Original papers report selected experimental and clinical investigations in all fields related to gynecology, obstetrics and reproduction. Short communications are published to allow immediate discussion of new data. The international and interdisciplinary character of this periodical provides an avenue to less accessible sources and to worldwide research for investigators and practitioners.
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