10 kHz spinal cord stimulation improves metrics of spinal sensory processing in a male STZ rat model of diabetes

IF 2.5 4区 医学 Q3 NEUROSCIENCES Neuroscience Letters Pub Date : 2024-09-14 DOI:10.1016/j.neulet.2024.137990
Dong Wang, Kwan Yeop Lee, Dongchul Lee, Zachary B Kagan, Kerry Bradley
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Abstract

To explore why clinical 10 kHz spinal cord stimulation (10 kHz SCS) might improve neurological function in a model of painful diabetic neuropathy (PDN), the short-term behavioral, electrophysiological, and histological effects of 10 kHz SCS were studied using adult male streptozotocin (STZ)-induced diabetic Sprague-Dawley rats. Four testing groups were established: Naïve controls (N = 8), STZ controls (N = 7), STZ+Sham SCS (N = 9), and STZ+10 kHz SCS (N = 11). After intraperitoneal injection (60 mg/kg) of STZ caused the rats to become hyperglycemic, SCS electrodes were implanted in the dorsal epidural space over the L5-L6 spinal segments in the STZ+Sham SCS and STZ+10 kHz SCS groups and were stimulated for 14 days. The von Frey filament paw withdrawal threshold was measured weekly. At termination, animals were anesthetized and the electrophysiologic response of dorsal horn neurons (receptive field size, vibration, radiant warmth) of the ipsilateral foot was measured. Tissue from the plantar paw surface was obtained post-euthanization for intraepidermal nerve fiber density measurements. In comparison to other control groups, while no significant effect of 10 kHz SCS on peripheral intraepidermal nerve fiber density was observed, 10 kHz SCS ‘normalized’ the central neural response to vibration, receptive field, and paw withdrawal threshold, and elevated the neural response to tissue recovery from warm stimuli. These results suggest that short-term, low intensity 10 kHz SCS operates in the spinal cord to ameliorate compromised sensory processing, and may compensate for reduced peripheral sensory functionality from chronic hyperglycemia, thereby treating a broader spectrum of the sensory symptoms in diabetic neuropathy.

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10 千赫脊髓刺激改善雄性 STZ 糖尿病大鼠脊髓感觉处理指标
为了探索临床 10 kHz 脊髓刺激(10 kHz SCS)为何能改善疼痛性糖尿病神经病变(PDN)模型的神经功能,我们使用成年雄性链脲佐菌素(STZ)诱导的糖尿病 Sprague-Dawley 大鼠研究了 10 kHz SCS 的短期行为、电生理学和组织学效应。共设立了四个测试组:原始对照组(8 只)、STZ 对照组(7 只)、STZ+Sham SCS(9 只)和 STZ+10 kHz SCS(11 只)。STZ+Sham SCS组和STZ+10 kHz SCS组大鼠腹腔注射(60 mg/kg)STZ导致高血糖后,在L5-L6脊柱节段的背侧硬膜外腔植入SCS电极,刺激14天。每周测量一次 Von Frey 丝爪退出阈值。结束时,对动物进行麻醉,并测量同侧足背角神经元的电生理反应(感受野大小、振动、辐射热)。动物安乐死后从跖掌表面获取组织,用于表皮内神经纤维密度测定。与其他对照组相比,虽然 10 kHz SCS 对外周表皮内神经纤维密度没有显著影响,但 10 kHz SCS 使中枢神经对振动、感受野和爪退缩阈值的反应 "正常化",并提高了神经对组织从温暖刺激中恢复的反应。这些结果表明,短期、低强度的 10 kHz SCS 可在脊髓发挥作用,改善受损的感觉处理功能,并可补偿因慢性高血糖导致的外周感觉功能减退,从而治疗糖尿病神经病变的各种感觉症状。
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来源期刊
Neuroscience Letters
Neuroscience Letters 医学-神经科学
CiteScore
5.20
自引率
0.00%
发文量
408
审稿时长
50 days
期刊介绍: Neuroscience Letters is devoted to the rapid publication of short, high-quality papers of interest to the broad community of neuroscientists. Only papers which will make a significant addition to the literature in the field will be published. Papers in all areas of neuroscience - molecular, cellular, developmental, systems, behavioral and cognitive, as well as computational - will be considered for publication. Submission of laboratory investigations that shed light on disease mechanisms is encouraged. Special Issues, edited by Guest Editors to cover new and rapidly-moving areas, will include invited mini-reviews. Occasional mini-reviews in especially timely areas will be considered for publication, without invitation, outside of Special Issues; these un-solicited mini-reviews can be submitted without invitation but must be of very high quality. Clinical studies will also be published if they provide new information about organization or actions of the nervous system, or provide new insights into the neurobiology of disease. NSL does not publish case reports.
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