Bacillus velezensis GX1 and its potential for the control of lily bulb rot

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biological Control Pub Date : 2024-09-16 DOI:10.1016/j.biocontrol.2024.105616

Sha Zhang , Ting Lou , Si Wu , Shenggui Liu , Hao Pan , Jing Yang , Yifeng Su , Wang Hu , Wei Xiang , Shuangyan Pan , Lei Sun

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Abstract

Bulb rot of lily (Lilium brownii var. viridulum), caused by Fusarium commune, is a serious soilborne disease of lily plants. In this study, several bacterial isolates were obtained from the rhizosphere of healthy lily plants and evaluated for biocontrol potential. Among the obtained isolates, GX1 had the highest level of antifungal activity in vitro, inhibiting the growth of F. commune by 52.0 %, relative to the control (CK). The isolate was identified as Bacillus velezensis based on its morphology and 16S rRNA and gyrB gene sequence analysis. The fermentation broth of GX1 after 12 d of fermentation at 25 °C (pH 7) exhibited strong antifungal activity. A crude lipopeptide extracts of GX1 fermentation broth was as effective (50.3 %) at inhibiting the mycelial growth of F. commune as living cultures of GX1. GX1 possesses fenD, bmyB, srfAA and bacA antimicrobial peptide (AMP) genes, which may be the primary source of its antifungal activity. GX1 significantly reduced the disease severity index of lily bulb rot in pot experiments, indicating that B. velezensis GX1 has substantial potential for the biological control of lily bulb rot.

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韦氏芽孢杆菌 GX1 及其防治百合球茎腐烂病的潜力

百合（Lilium brownii var. viridulum）鳞茎腐烂病是由共生镰刀菌（Fusarium commune）引起的，是百合植物的一种严重土传病害。本研究从健康百合植物的根瘤层中获得了几种细菌分离物，并对其生物防治潜力进行了评估。在获得的分离物中，GX1 的体外抗真菌活性最高，与对照组（CK）相比，它对 F. commune 的生长抑制率达 52.0%。根据其形态、16S rRNA 和 gyrB 基因序列分析，该分离物被鉴定为 Velezensis 杆菌。在 25 °C（pH 值为 7）下发酵 12 d 后，GX1 的发酵液表现出很强的抗真菌活性。从 GX1 发酵液中提取的粗脂肽抑制 F. commune 菌丝生长的效果（50.3%）与 GX1 活体培养物的效果相当。GX1 具有 fenD、bmyB、srfAA 和 bacA 抗菌肽（AMP）基因，这可能是其抗真菌活性的主要来源。在盆栽实验中，GX1 能明显降低百合球茎腐烂病的病情严重指数，这表明 B. velezensis GX1 在百合球茎腐烂病的生物防治方面具有巨大潜力。

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.