Evaluating the Effects of Solid-Phase Cartridge Chemistry on Extraction of Bisphosphonates and ITPP From Equine Plasma.

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS Drug Testing and Analysis Pub Date : 2024-09-16 DOI:10.1002/dta.3800

Joshua Klingberg, Stacey Richards, Travis Hochwallner, Lauren Kennan, John Keledjian

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Abstract

Bisphosphonate drugs and myo-inositol trispyrophosphate are of concern to the racing industry and have been listed as prohibited substances in equine sports. The current bisphosphonate plasma screening analysis employed at the Australian Racing Forensic Laboratory involves the use of sequential solid-phase extraction procedures, passing the samples through a mixed mode cartridge, followed by a weak anion exchange cartridge. The eluates collected following the second extraction are then methylated and analysed by liquid chromatography-mass spectrometry. Under these extraction conditions, some bisphosphonates have shown poor recovery. To improve the extraction efficacy, the effects of cartridge chemistry were evaluated. In particular, the weak anion exchange cartridges used for screening were compared to an affinisep AttractSPE polymeric phase cartridge. The effectiveness of each extraction approach was assessed through both a visual comparison of signal to noise in extracted chromatograms and recovery measurements to determine the best approach for routine screening.

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评估固相滤芯化学法对从马血浆中提取双膦酸盐和 ITPP 的影响。

双膦酸盐药物和肌醇三异焦磷酸盐是赛马业关注的问题，已被列为马术运动的禁用物质。澳大利亚赛马法医实验室目前采用的双膦酸盐血浆筛查分析方法包括使用顺序固相萃取程序，将样品通过混合模式滤芯，然后再通过弱阴离子交换滤芯。第二次萃取后收集的洗脱液经过甲基化处理，然后用液相色谱-质谱法进行分析。在这种萃取条件下，某些双膦酸盐的回收率较低。为了提高萃取效率，我们对滤芯化学成分的影响进行了评估。特别是将用于筛选的弱阴离子交换柱与 affinisep AttractSPE 聚合相柱进行了比较。通过对萃取色谱中的信噪比和回收率进行直观比较，评估了每种萃取方法的效果，以确定用于常规筛选的最佳方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.