Characterization of Exosomes Released from Mycobacterium abscessus-Infected Macrophages

IF 3.4 4区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS Proteomics Pub Date : 2024-09-16 DOI:10.1002/pmic.202400181
Charlie A. Vermeire, Xuejuan Tan, Aidaly Ramos-Leyva, Ava Wood, Stephen K. Kotey, Steven D. Hartson, Yurong Liang, Lin Liu, Yong Cheng
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Abstract

Extracellular vesicles (EVs), such as exosomes, play a critical role in cell-to-cell communication and regulating cellular processes in recipient cells. Non-tuberculous mycobacteria (NTM), such as Mycobacterium abscessus, are a group of environmental bacteria that can cause severe lung infections in populations with pre-existing lung conditions, such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). There is limited knowledge of the engagement of EVs in the host–pathogen interactions in the context of NTM infections. In this study, we found that M. abscessus infection increased the release of a subpopulation of exosomes (CD9, CD63, and/or CD81 positive) by mouse macrophages in cell culture. Proteomic analysis of these vesicles demonstrated that M. abscessus infection affects the enrichment of host proteins in exosomes released by macrophages. When compared to exosomes from uninfected macrophages, exosomes released by M. abscessus-infected macrophages significantly improved M. abscessus growth and downregulated the intracellular level of glutamine in recipient macrophages in cell culture. Increasing glutamine concentration in the medium rescued intracellular glutamine levels and M. abscessus killing in recipient macrophages that were treated with exosomes from M. abscessus-infected macrophages. Taken together, our results indicate that exosomes may serve as extracellular glutamine eliminators that interfere with glutamine-dependent M. abscessus killing in recipient macrophages.

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受脓肿分枝杆菌感染的巨噬细胞释放的外泌体的特征。
细胞外小泡(EVs),如外泌体,在细胞间通信和调节受体细胞的细胞过程中发挥着至关重要的作用。非结核分枝杆菌(NTM),如脓肿分枝杆菌,是一类环境细菌,可在已有肺部疾病(如囊性纤维化(CF)和慢性阻塞性肺病(COPD))的人群中引起严重的肺部感染。在非结核杆菌感染的情况下,人们对EVs参与宿主与病原体相互作用的了解十分有限。在这项研究中,我们发现脓肿霉菌感染会增加细胞培养中小鼠巨噬细胞释放的外泌体亚群(CD9、CD63 和/或 CD81 阳性)。对这些囊泡进行的蛋白质组学分析表明,脓肿病菌感染会影响巨噬细胞释放的外泌体中宿主蛋白质的富集。与未感染的巨噬细胞释放的外泌体相比,脓肿病菌感染的巨噬细胞释放的外泌体能显著改善脓肿病菌的生长,并降低细胞培养中受体巨噬细胞内谷氨酰胺的水平。增加培养基中谷氨酰胺的浓度可挽救细胞内谷氨酰胺的水平,以及用受脓肿感染的巨噬细胞释放的外泌体处理的受体巨噬细胞对脓肿病毒的杀伤力。综上所述,我们的研究结果表明,外泌体可作为细胞外谷氨酰胺消除剂,干扰受体巨噬细胞中谷氨酰胺依赖性的脓毒症噬菌体杀伤作用。
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来源期刊
Proteomics
Proteomics 生物-生化研究方法
CiteScore
6.30
自引率
5.90%
发文量
193
审稿时长
3 months
期刊介绍: PROTEOMICS is the premier international source for information on all aspects of applications and technologies, including software, in proteomics and other "omics". The journal includes but is not limited to proteomics, genomics, transcriptomics, metabolomics and lipidomics, and systems biology approaches. Papers describing novel applications of proteomics and integration of multi-omics data and approaches are especially welcome.
期刊最新文献
Front Cover Editorial Board: Proteomics 5–6'25 Contents: Proteomics 5–6'25 Standard abbreviations Development and Validation of a Capillary Electrophoresis Coupled to Mass Spectrometry Pipeline for Comparable Assessment of the Plasma Peptidome.
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