Zona Incerta GABAergic Neurons Facilitate Emergence from Isoflurane Anesthesia in Mice

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Neurochemical Research Pub Date : 2024-09-23 DOI:10.1007/s11064-024-04230-9
Hong Chen, Chengxi Liu, Junxiao Liu, Chengdong Yuan, Haifeng He, Yu Zhang, Shouyang Yu, Tianyuan Luo, Wei Shen, Tian Yu
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Abstract

The zona incerta (ZI) predominantly consists of gamma-aminobutyric acid (GABAergic) neurons, located adjacent to the lateral hypothalamus. GABA, acting on GABAA receptors, serves as a crucial neuromodulator in the initiation and maintenance of general anesthesia. In this study, we aimed to investigate the involvement of ZI GABAergic neurons in the general anesthesia process. Utilizing in-vivo calcium signal optical fiber recording, we observed a decrease in the activity of ZI GABAergic neurons during isoflurane anesthesia, followed by a significant increase during the recovery phase. Subsequently, we selectively ablated ZI GABAergic neurons to explore their role in general anesthesia, revealing no impact on the induction of isoflurane anesthesia but a prolonged recovery time, accompanied by a reduction in delta-band power in mice under isoflurane anesthesia. Finally, through optogenetic activation/inhibition of ZI GABAergic neurons during isoflurane anesthesia, we discovered that activation of these neurons facilitated emergence without affecting the induction process, while inhibition delayed emergence, leading to fluctuations in delta band activity. In summary, these findings highlight the involvement of ZI GABAergic neurons in modulating the emergence of isoflurane anesthesia.

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昏迷区 GABA 能神经元有助于小鼠从异氟醚麻醉中苏醒。
内侧区(ZI)主要由γ-氨基丁酸(GABA)能神经元组成,毗邻外侧下丘脑。GABA 作用于 GABAA 受体,是启动和维持全身麻醉的重要神经调节剂。本研究旨在探讨ZI GABA能神经元在全身麻醉过程中的参与情况。通过体内钙信号光纤记录,我们观察到在异氟烷麻醉过程中,ZI GABA能神经元的活性下降,随后在恢复阶段显著上升。随后,我们选择性地消融了ZI GABA能神经元,以探索它们在全身麻醉中的作用,结果显示,消融ZI GABA能神经元对异氟醚麻醉的诱导没有影响,但恢复时间延长,同时异氟醚麻醉下小鼠的δ波段功率降低。最后,通过在异氟烷麻醉期间对 ZI GABA 能神经元进行光遗传学激活/抑制,我们发现这些神经元的激活可促进苏醒而不影响诱导过程,而抑制则会延迟苏醒,从而导致δ波段活动的波动。总之,这些发现强调了ZI GABA能神经元参与调节异氟烷麻醉的唤醒。
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来源期刊
Neurochemical Research
Neurochemical Research 医学-神经科学
CiteScore
7.70
自引率
2.30%
发文量
320
审稿时长
6 months
期刊介绍: Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.
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