New Insight in Using of Mesenchyme Stem Cell Conditioning Medium for the Impaired Muscle related Biomarkers: In vivo Study with Rat Model.

IF 0.6 Q3 MEDICINE, GENERAL & INTERNAL Annals of African Medicine Pub Date : 2024-10-01 Epub Date: 2024-09-14 DOI:10.4103/aam.aam_205_23

Ronald Winardi Kartika, Veronika Maria Sidharta, Tena Djuartina, Cynthia Retna Sartika, Kris Herawan Timotius

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Abstract

Aims and objectives: This study aimed to investigate the effects of Umbilical Cord Mesencymal Stem Cell Conditioning Medium (UC MSC-CM) administration on body weight recovery and the level of four molecular biomarkers, namely Superoxide Dismutase (SOD), vascular Endothelial Growth Factor (VEGF), C-Reactive Protein (CRP), and myostatin.

Materials and methods: Secretome was injected intramuscularly twice at 1.5 mL (day 7 and 14) into the right thigh of high-dose, short-term galactose-induced aging rats. The data of day 7 (before) and day 21 (after the administration) were evaluated. The body weights and the four biomarkers were measured before (day 7) and after intervention (day 21).

Results: This study showed that the UC MSC-CM intramuscular administrations did not influence body weight regeneration. However, it could increase SOD and VEGF levels and decrease CRP and myostatin levels.

Conclusion: Treatment with UC MSC-CM is a promising and potential agent in treating sarcopenia.

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使用间充质干细胞调节介质治疗肌肉相关生物标志物损伤的新见解：大鼠模型体内研究

目的和目标：本研究旨在探讨脐带间充质干细胞调理液（UC MSC-CM）对体重恢复和四种分子生物标志物（即超氧化物歧化酶（SOD）、血管内皮生长因子（VEGF）、C-反应蛋白（CRP）和肌生长因子）水平的影响：向高剂量、短期半乳糖诱导的衰老大鼠的右大腿肌肉注射两次分泌物，每次 1.5 mL（第 7 天和第 14 天）。评估第 7 天（给药前）和第 21 天（给药后）的数据。结果：结果：研究表明，UC间充质干细胞-间充质干细胞肌肉注射不会影响体重再生。结论：使用 UC 间充质干细胞进行治疗不会影响体重再生，但可提高 SOD 和血管内皮生长因子水平，降低 CRP 和肌生成蛋白水平：结论：使用 UC 间充质干细胞治疗肌少症是一种很有前景和潜力的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Annals of African Medicine MEDICINE, GENERAL & INTERNAL-

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0.90

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期刊介绍： The Annals of African Medicine is published by the Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria and the Annals of African Medicine Society. The Journal is intended to serve as a medium for the publication of research findings in the broad field of Medicine in Africa and other developing countries, and elsewhere which have relevance to Africa. It will serve as a source of information on the state of the art of Medicine in Africa, for continuing education for doctors in Africa and other developing countries, and also for the publication of meetings and conferences. The journal will publish articles I any field of Medicine and other fields which have relevance or implications for Medicine.