Impact of prematurity on LINE-1 promoter methylation.

IF 3 4区医学 Q2 GENETICS & HEREDITY Epigenomics Pub Date : 2024-01-01 Epub Date: 2024-09-19 DOI:10.1080/17501911.2024.2397329

Paulo Victor Barbosa Eleutério Dos Santos, Aline de Araújo Brasil, Leo Travassos Vieira Milone, Georgia Chalfun, Stephanie Cristina Alves de Oliveira Saide, Margarida Dos Santos Salú, Mariana Barros Genuíno de Oliveira, Jaqueline Rodrigues Robaina, Fernanda Lima-Setta, Gustavo Rodrigues-Santos, Maria Clara de Magalhães-Barbosa, Antônio José Ledo Alves da Cunha, Arnaldo Prata-Barbosa

{"title":"Impact of prematurity on LINE-1 promoter methylation.","authors":"Paulo Victor Barbosa Eleutério Dos Santos, Aline de Araújo Brasil, Leo Travassos Vieira Milone, Georgia Chalfun, Stephanie Cristina Alves de Oliveira Saide, Margarida Dos Santos Salú, Mariana Barros Genuíno de Oliveira, Jaqueline Rodrigues Robaina, Fernanda Lima-Setta, Gustavo Rodrigues-Santos, Maria Clara de Magalhães-Barbosa, Antônio José Ledo Alves da Cunha, Arnaldo Prata-Barbosa","doi":"10.1080/17501911.2024.2397329","DOIUrl":null,"url":null,"abstract":"Aim: Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.Materials & methods: Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.Results: Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.Conclusion: This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":" ","pages":"1253-1264"},"PeriodicalIF":3.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486321/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/17501911.2024.2397329","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/19 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.Materials & methods: Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.Results: Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.Conclusion: This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

早产对 LINE-1 启动子甲基化的影响

目的：LINE-1的启动子甲基化可能受到早产儿的影响，但文献中的证据很少：对早产儿和足月新生儿第0、5、30和90天的血液进行亚硫酸氢盐转化和热测序，分析LINE-1启动子区域的DNA甲基化百分比：结果：早产儿整体上在出生时甲基化率明显较低，但随着时间的推移，这种差异逐渐缩小。然而，极早产儿亚组（结论：该研究强调了早产儿甲基化的关键作用：这项研究强调了早产对 LINE-1 甲基化模式的关键作用。这些发现强调了早产儿表观遗传调控的复杂性，并强调了进一步研究的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.