Agar lot-specific inhibition in the plating efficiency of yeast spores and cells.

IF 2.1 3区 生物学 Q3 GENETICS & HEREDITY G3: Genes|Genomes|Genetics Pub Date : 2024-09-23 DOI:10.1093/g3journal/jkae229
Reine U Protacio, Mari K Davidson, Emory G Malone, Dominique Helmlinger, Jeremy R Smith, Patrick A Gibney, Wayne P Wahls
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Abstract

The fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae are highly diverged (530 mya), single-celled, model eukaryotic organisms. Scientists employ mating, meiosis, and the plating of ascospores and cells to generate strains with novel genotypes and to discover biological processes. Our three laboratories encountered independently sudden-onset, major impediments to such research. Spore suspensions and vegetative cells no longer plated effectively on minimal media. By systematically analyzing multiple different media components from multiple different suppliers, we identified the source of the problem. Specific lots of agar were toxic. We report that this sporadic toxicity affects independently the agar stocks of multiple vendors, has occurred repeatedly over at least three decades, and extends to species in highly diverged taxa. Interestingly, the inhibitory effects displayed variable penetrance and were attenuated on rich media. Consequently, quality control checks that use only rich media can provide false assurances on the quality of the agar. Lastly, we describe likely sources of the toxicity and we provide specific guidance for quality control measures that should be applied by all vendors as preconditions for their sale of agar.

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琼脂批次对酵母孢子和细胞培养效率的抑制作用。
裂殖酵母(Schizosaccharomyces pombe)和酿酒酵母(Saccharomyces cerevisiae)是高度分化(530 万年前)的单细胞模式真核生物。科学家们通过交配、减数分裂以及分离酵母孢子和细胞来产生具有新基因型的菌株,并发现生物过程。我们的三个实验室在进行此类研究时都遇到了突如其来的重大障碍。孢子悬浮液和无性细胞不再能有效地在最小培养基上培养。通过系统分析来自多个不同供应商的多种不同培养基成分,我们找到了问题的根源。特定批次的琼脂是有毒的。我们报告说,这种零星的毒性影响到多个供应商的琼脂存货,至少在三十年内反复出现,并延伸到高度分化的类群中的物种。有趣的是,这种抑制作用具有不同的渗透性,在富集培养基上会减弱。因此,仅使用富集培养基进行质量控制检查可能会对琼脂的质量造成错误的保证。最后,我们描述了毒性的可能来源,并为质量控制措施提供了具体指导,所有供应商都应将这些措施作为销售琼脂的前提条件。
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来源期刊
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics GENETICS & HEREDITY-
CiteScore
5.10
自引率
3.80%
发文量
305
审稿时长
3-8 weeks
期刊介绍: G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.
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