Temporal changes in the mouse gut bacteriota influenced by host sex, diet, and exercise.

IF 3.3 3区 医学 Q1 PHYSIOLOGY Journal of applied physiology Pub Date : 2024-11-01 Epub Date: 2024-09-19 DOI:10.1152/japplphysiol.00487.2023
Robert A Dowden, Lee J Kerkhof, Paul J Wisniewski, Max M Häggblom, Sara C Campbell
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Abstract

The gut microbiota plays an important role in host physiology. However, the effects of host sex, lifestyle, and temporal influences on the bacterial community within the gut remain ill-defined. To address this gap, we evaluated 56 male and female mice over a 10-wk study to assess the effects of sex, diet, and exercise on gut community dynamics. Mice were randomly assigned to high-fat or control diet feeding and had free access to running wheels or remained sedentary throughout the study period. The fecal bacterial community was characterized by rRNA operon profiling via nanopore sequencing. Differential abundance testing indicated that ∼200 bacterial taxa were significantly influenced by sex, diet, or exercise (4.2% of total community), which also changed over time (82 taxa, 1.7% of total community). Phylogenetic analysis of taxa closely related to Dysosmobacter welbionis, and several members of the family Muribaculaceae were examined more closely and demonstrated distinct species/strain level subclustering by host sex, diet, and exercise. Collectively, these data suggest that sex and lifestyle can alter the gut bacteriota at the species/strain level that may play a role in host health. These results also highlight the need for improved characterization methods to survey microbial communities at finer taxonomic resolution.NEW & NOTEWORTHY This study demonstrates that host sex, diet, and exercise can each modulate gut bacterial community structure, which may have consequences to host physiology. Our analysis shows selection of novel strains and genera for some members of the Oscillospiraceae and Muribaculaceae by host sex, diet, and activity status. Overall, these findings provide a framework for detecting the next generation of beneficial bacteria targeting obesity and associated metabolic diseases in a sex-specific manner.

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小鼠肠道细菌群受宿主性别、饮食和运动影响的时间变化
肠道微生物群对宿主的生理起着重要作用。然而,宿主的性别、生活方式和时间对肠道内细菌群落的影响仍不明确。为了填补这一空白,我们对 56 只雌雄小鼠进行了为期 10 周的研究,以评估性别、饮食和运动对肠道群落动态的影响。在整个研究期间,小鼠被随机分配到高脂肪或对照组饮食喂养,并可自由使用跑步轮或保持静止不动。通过纳米孔测序技术对粪便细菌群落进行了rRNA操作子扩增片段分析。差异丰度测试表明,约有 200 个细菌类群受到性别、饮食或运动的显著影响(占群落总数的 4.2%),这些类群也随着时间的推移而变化(82 个类群,占群落总数的 1.7%)。对与韦尔比恩斯痢疾杆菌密切相关的类群和穆里巴库尔科(Muribaculaceae)的几个成员进行了更仔细的系统发育分析,结果表明宿主性别、饮食和运动对物种/菌株产生了不同程度的亚群集。总之,这些数据表明,性别和生活方式会在物种/菌株水平上改变肠道细菌群,这可能会对宿主的健康产生影响。这些结果还突出表明,需要改进表征方法,以更精细的分类分辨率调查微生物群落。
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来源期刊
CiteScore
6.00
自引率
9.10%
发文量
296
审稿时长
2-4 weeks
期刊介绍: The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.
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