CATSPER2 and SPEF2 are potential molecular markers for boar sperm quality: a population association study.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-09-23 DOI:10.1007/s10815-024-03252-0

Zulfiqar Ahmed, Min Liu, Shuntao Huang, Dequan Xu

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Abstract

Purpose: This study investigates the role of cation channel sperm associated 2 (CATSPER2) and sperm flagella 2 (SPEF2) genes in boar spermatogenesis, focusing on their association with sperm quality traits in boars.

Methods: Utilizing targeted next-generation sequencing, we identified and genotyped two polymorphisms in CATSPER2 (rs341636020G > A, rs326912346G > T) and three variants in SPEF2 (rs320839956A > G, rs334209514C > A, rs325319860C > T) across three boar breeds (Duroc, n = 181; Landrace, n = 87; Large White, n = 52).

Results: Our results confirmed the presence of the specified single nucleotide polymorphisms (SNPs), adhering to association study criteria. In CATSPER2, significant associations were detected between rs341636020G > A and sperm curvilinear velocity (VCL) in Duroc and Landrace boars, and between rs326912346G > T and straight velocity (VSL) in Duroc and Large White boars. For SPEF2, rs320839956A > G was significantly linked to sperm viability in Duroc and Landrace and to sperm concentration (SCON) in Large White boars. Additionally, rs334209514C > A and rs325319860C > T showed significant associations with SCON and VCL respectively, in Doruc and Landrace boars.

Conclusions: Overall, our findings suggest that CATSPER2 and SPEF2 SNPs significantly impact boar sperm quality traits. These genetic markers have the potential to enhance boar fertility through selective breeding programs, contributing to the optimization of reproductive performance in pigs.

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CATSPER2 和 SPEF2 是公猪精子质量的潜在分子标记：一项群体关联研究。

目的：本研究探讨了阳离子通道精子相关2（CATSPER2）和精子鞭毛2（SPEF2）基因在公猪精子发生中的作用，重点研究了它们与公猪精子质量性状的关系：利用定向下一代测序技术，我们在三个公猪品种（杜洛克公猪，n = 181；陆地公猪，n = 87；大白公猪，n = 52）中发现并对 CATSPER2 的两个多态性（rs341636020G > A、rs326912346G > T）和 SPEF2 的三个变异（rs320839956A > G、rs334209514C > A、rs325319860C > T）进行了基因分型：我们的研究结果证实了特定单核苷酸多态性（SNPs）的存在，符合关联研究标准。在 CATSPER2 中，发现 rs341636020G > A 与杜洛克公猪和陆种公猪的精子曲线速度（VCL）有显著关联，rs326912346G > T 与杜洛克公猪和大白公猪的精子直线速度（VSL）有显著关联。就 SPEF2 而言，rs320839956A > G 与杜洛克公猪和兰色公猪的精子活力以及大白公猪的精子浓度 (SCON) 显著相关。此外，rs334209514C > A 和 rs325319860C > T 分别与多鲁克公猪和兰德瑞斯公猪的 SCON 和 VCL 有显著关联：总之，我们的研究结果表明，CATSPER2 和 SPEF2 SNPs 对公猪精子质量性状有显著影响。这些遗传标记具有通过选择性育种计划提高公猪繁殖力的潜力，有助于优化猪的繁殖性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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