First report of a carbapenem-resistant Aeromonas veronii environmental isolate in the United States co-harboring two carbapenemase genes

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-09-11 DOI:10.1016/j.jgar.2024.08.010
{"title":"First report of a carbapenem-resistant Aeromonas veronii environmental isolate in the United States co-harboring two carbapenemase genes","authors":"","doi":"10.1016/j.jgar.2024.08.010","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>The spread of carbapenem-resistant bacteria (CRB), and especially carbapenemase-producing CRB, is a global public health threat. Among them, <em>Aeromonas</em> species are of increasing concern because these emerging opportunistic pathogens are widespread in the environment and have increasingly been found to be resistant to carbapenems. The aim of this study was to investigate the genome and carbapenem-resistance determinants of <em>Aeromonas veronii</em> SS-M2–3, a highly carbapenem-resistant, carbapenemase-producing, river isolate from California (U.S.).</div></div><div><h3>Methods</h3><div>We first used disk diffusion assays to characterize the susceptibility profile to carbapenems and other antibiotics of <em>A. veronii</em> SS-M2–3. We next used whole-genome sequencing using the Illumina platform and bioinformatics analysis to characterize the resistome of this isolate and identify its carbapenemase genes.</div></div><div><h3>Results</h3><div><em>A. veronii</em> SS-M2–3 was resistant to all carbapenems tested and amoxicillin-clavulanic acid, whereas it was sensitive to cefotaxime and all non-β-lactam antibiotics tested. Whole genome sequencing of this isolate revealed a complex resistome that included multidrug efflux pump genes and three chromosomal β-lactamase genes. These three genes encoded for highly conserved variants (82% to 97% amino acid identity) of the ChpA3 subclass B2 metallo-carbapenemase, OXA-12 class D carbapenemase and the FOX-2 class C β-lactamase. This is the first report of an environmental <em>A. veronni</em> isolate from the U.S. co-harbouring two carbapenemase genes.</div></div><div><h3>Conclusions</h3><div>These findings reveal that natural aquatic environments in the U.S. represent an underappreciated reservoir of carbapenem-resistant <em>Aeromonas veronii</em> isolates that can carry multiple carbapenemase genes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001644","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Objectives

The spread of carbapenem-resistant bacteria (CRB), and especially carbapenemase-producing CRB, is a global public health threat. Among them, Aeromonas species are of increasing concern because these emerging opportunistic pathogens are widespread in the environment and have increasingly been found to be resistant to carbapenems. The aim of this study was to investigate the genome and carbapenem-resistance determinants of Aeromonas veronii SS-M2–3, a highly carbapenem-resistant, carbapenemase-producing, river isolate from California (U.S.).

Methods

We first used disk diffusion assays to characterize the susceptibility profile to carbapenems and other antibiotics of A. veronii SS-M2–3. We next used whole-genome sequencing using the Illumina platform and bioinformatics analysis to characterize the resistome of this isolate and identify its carbapenemase genes.

Results

A. veronii SS-M2–3 was resistant to all carbapenems tested and amoxicillin-clavulanic acid, whereas it was sensitive to cefotaxime and all non-β-lactam antibiotics tested. Whole genome sequencing of this isolate revealed a complex resistome that included multidrug efflux pump genes and three chromosomal β-lactamase genes. These three genes encoded for highly conserved variants (82% to 97% amino acid identity) of the ChpA3 subclass B2 metallo-carbapenemase, OXA-12 class D carbapenemase and the FOX-2 class C β-lactamase. This is the first report of an environmental A. veronni isolate from the U.S. co-harbouring two carbapenemase genes.

Conclusions

These findings reveal that natural aquatic environments in the U.S. represent an underappreciated reservoir of carbapenem-resistant Aeromonas veronii isolates that can carry multiple carbapenemase genes.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
首次报告美国环境中分离出的对碳青霉烯类耐药的维龙单胞菌同时携带两种碳青霉烯酶基因。
目标:耐碳青霉烯类细菌(CRB),尤其是产生碳青霉烯类酶的 CRB 的传播是对全球公共卫生的威胁。其中,气单胞菌日益受到关注,因为这些新出现的机会性病原体在环境中广泛存在,而且越来越多地被发现对碳青霉烯类产生耐药性。本研究的目的是调查维龙单胞菌 SS-M2-3 的基因组和碳青霉烯类耐药性决定因素:方法:我们首先使用磁盘扩散法分析了维罗纳弧菌 SS-M2-3 对碳青霉烯类和其他抗生素的敏感性。接下来,我们使用 Illumina 平台进行全基因组测序,并进行生物信息学分析,以确定该分离株的耐药基因组特征并鉴定其碳青霉烯酶基因:结果:A. veronii SS-M2-3对所测试的所有碳青霉烯类抗生素和阿莫西林-克拉维酸均耐药,而对头孢他啶和所测试的所有非β-内酰胺类抗生素均敏感。对该分离株的全基因组测序发现了一个复杂的抗药性基因组,其中包括多药外排泵基因和三个染色体β-内酰胺酶基因。这三个基因编码 ChpA3 B2 亚类金属碳青霉烯酶、OXA-12 D 类碳青霉烯酶和 FOX-2 C 类 β-内酰胺酶的高度保守变体(82%-97% 氨基酸同一性)。这是首次报道美国环境中的维龙尼甲虫分离物同时携带两种碳青霉烯酶基因:结论:这些研究结果表明,美国的自然水生环境是一个未得到充分重视的耐碳青霉烯类的维龙单胞菌分离物库,这些分离物可携带多种碳青霉烯类酶基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
期刊最新文献
Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli. From Forgotten Cure to Modern Medicine: The Resurgence of Bacteriophage Therapy. Rapid immunochromatographic detection of carbapenemases directly from positive blood cultures in patients colonized by carbapenemase-producing bacteria. Study of the mechanisms of heteroresistance to colistin in a strain of Enterobacter cloacae by random mutagenesis. Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1