Electrophysiological evaluation of the effect of peptide toxins on voltage-gated ion channels: a scoping review on theoretical and methodological aspects with focus on the Central and South American experience.

IF 1.8 3区 医学 Q4 TOXICOLOGY Journal of Venomous Animals and Toxins Including Tropical Diseases Pub Date : 2024-09-02 eCollection Date: 2024-01-01 DOI:10.1590/1678-9199-JVATITD-2023-0048
Jessica Rojas-Palomino, Alejandro Gómez-Restrepo, Cristian Salinas-Restrepo, César Segura, Marco A Giraldo, Juan C Calderón
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Abstract

The effect of peptide toxins on voltage-gated ion channels can be reliably assessed using electrophysiological assays, such as the patch-clamp technique. However, much of the toxinological research done in Central and South America aims at purifying and characterizing biochemical properties of the toxins of vegetal or animal origin, lacking electrophysiological approaches. This may happen due to technical and infrastructure limitations or because researchers are unfamiliar with the techniques and cellular models that can be used to gain information about the effect of a molecule on ion channels. Given the potential interest of many research groups in the highly biodiverse region of Central and South America, we reviewed the most relevant conceptual and methodological developments required to implement the evaluation of the effect of peptide toxins on mammalian voltage-gated ion channels using patch-clamp. For that, we searched MEDLINE/PubMed and SciELO databases with different combinations of these descriptors: "electrophysiology", "patch-clamp techniques", "Ca2+ channels", "K+ channels", "cnidarian venoms", "cone snail venoms", "scorpion venoms", "spider venoms", "snake venoms", "cardiac myocytes", "dorsal root ganglia", and summarized the literature as a scoping review. First, we present the basics and recent advances in mammalian voltage-gated ion channel's structure and function and update the most important animal sources of channel-modulating toxins (e.g. cnidarian and cone snails, scorpions, spiders, and snakes), highlighting the properties of toxins electrophysiologically characterized in Central and South America. Finally, we describe the local experience in implementing the patch-clamp technique using two models of excitable cells, as well as the participation in characterizing new modulators of ion channels derived from the venom of a local spider, a toxins' source less studied with electrophysiological techniques. Fostering the implementation of electrophysiological methods in more laboratories in the region will strengthen our capabilities in many fields, such as toxinology, toxicology, pharmacology, natural products, biophysics, biomedicine, and bioengineering.

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肽毒素对电压门控离子通道影响的电生理学评估:以中美洲和南美洲的经验为重点,对理论和方法方面进行的范围审查。
多肽毒素对电压门控离子通道的影响可通过电生理学测定法(如贴片钳技术)进行可靠评估。然而,中美洲和南美洲所做的大部分毒素学研究都旨在纯化和描述植物或动物源毒素的生化特性,而缺乏电生理学方法。造成这种情况的原因可能是技术和基础设施的限制,也可能是研究人员不熟悉可用于获取分子对离子通道影响信息的技术和细胞模型。考虑到中美洲和南美洲生物多样性丰富地区的许多研究小组的潜在兴趣,我们回顾了使用膜片钳评估肽毒素对哺乳动物电压门控离子通道的影响所需的最相关的概念和方法发展。为此,我们在 MEDLINE/PubMed 和 SciELO 数据库中搜索了以下描述符的不同组合:"电生理学"、"膜片钳技术"、"Ca2+ 通道"、"K+ 通道"、"刺胞动物毒液"、"锥蜗牛毒液"、"蝎子毒液"、"蜘蛛毒液"、"蛇毒"、"心肌细胞"、"背根神经节",并以范围综述的形式对文献进行了总结。首先,我们介绍了哺乳动物电压门控离子通道结构和功能的基础知识和最新进展,并更新了通道调节毒素最重要的动物来源(如刺蛇和锥螺、蝎子、蜘蛛和蛇),重点介绍了在中美洲和南美洲电生理特征的毒素特性。最后,我们介绍了当地利用两种可兴奋细胞模型实施膜片钳技术的经验,以及参与鉴定从当地一种蜘蛛毒液中提取的离子通道新调节剂的情况。在该地区更多的实验室推广电生理方法将加强我们在毒理学、毒理学、药理学、天然产品、生物物理学、生物医学和生物工程等多个领域的能力。
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来源期刊
CiteScore
4.80
自引率
8.30%
发文量
39
审稿时长
6-12 weeks
期刊介绍: Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) is a non-commercial academic open access publication dedicated to research on all aspects of toxinology, venomous animals and tropical diseases. Its interdisciplinary content includes original scientific articles covering research on toxins derived from animals, plants and microorganisms. Topics of interest include, but are not limited to:systematics and morphology of venomous animals;physiology, biochemistry, pharmacology and immunology of toxins;epidemiology, clinical aspects and treatment of envenoming by different animals, plants and microorganisms;development and evaluation of antivenoms and toxin-derivative products;epidemiology, clinical aspects and treatment of tropical diseases (caused by virus, bacteria, algae, fungi and parasites) including the neglected tropical diseases (NTDs) defined by the World Health Organization.
期刊最新文献
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