PROTEIN ELECTROPHORESIS AND REFERENCE INTERVALS OF SERUM PROTEINS OF A CAPTIVE POPULATION OF ROTI ISLAND SNAKE-NECKED TURTLES (CHELODINA MCCORDI).

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES Journal of Zoo and Wildlife Medicine Pub Date : 2024-09-01 DOI:10.1638/2023-0104

Shin Min Chong, Guillaume Douay

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Abstract

Protein electrophoresis (PEP) is an important tool in mammals to characterize specific dysproteinemias and detect acute and chronic inflammatory responses. In reptiles, PEP is the gold standard method for globulin fraction determination and albumin measurement. In this study, preliminary reference intervals were established for serum PEP in 22 clinically healthy adult Roti Island snake-necked turtles (Chelodina mccordi), a critically endangered species, kept in captivity and sampled over two monsoon seasons. The species has a prominent prealbumin fraction and β-globulins were the dominant globulin fraction. Significant differences between females and males were found in prealbumin (P < 0.01), albumin (P = 0.02), α1-globulin (P = 0.05) and γ-globulin (P = 0.01). Gravid females had significantly lower total protein (P < 0.01), prealbumin (P < 0.01), albumin (P < 0.01) and albumin:globulin ratio (P = 0.01). These preliminary reference intervals should aid in clinical investigation in this species as well as further research studies seeking to understand the application of PEP in reptilian species.

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罗蒂岛蛇颈龟（Chelodina mccordi）圈养种群血清蛋白的蛋白质电泳和参考区间。

蛋白质电泳（PEP）是哺乳动物鉴定特异性蛋白异常和检测急性和慢性炎症反应的重要工具。在爬行动物中，PEP 是测定球蛋白组分和白蛋白的黄金标准方法。在这项研究中，对 22 只临床健康的成年罗蒂岛蛇颈龟（Chelodina mccordi）（一种极度濒危物种）的血清 PEP 建立了初步参考区间。该物种的前白蛋白部分很突出，β-球蛋白是主要的球蛋白部分。雌性和雄性在前白蛋白（P < 0.01）、白蛋白（P = 0.02）、α1-球蛋白（P = 0.05）和γ-球蛋白（P = 0.01）方面存在显著差异。妊娠女性的总蛋白（P < 0.01）、前白蛋白（P < 0.01）、白蛋白（P < 0.01）和白蛋白与球蛋白的比率（P = 0.01）均明显较低。这些初步参考区间应有助于该物种的临床调查以及进一步的研究，以了解 PEP 在爬行动物中的应用。

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来源期刊

Journal of Zoo and Wildlife Medicine 农林科学-兽医学

CiteScore

1.70

自引率

14.30%

发文量

审稿时长

9-24 weeks

期刊介绍： The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers. The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution. Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.