Isolation of a Moderately Acidophilic Nitrobacter from a Nitrifying Community Supplied with Urea.

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Microbes and Environments Pub Date : 2024-01-01 DOI:10.1264/jsme2.ME24027

Yuta Endo, Hirotsugu Fujitani, Ayano Kaneko, Takuya Ninomiya, Chiharu Umezawa, Megumi Kuroiwa, Yuichi Suwa

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Abstract

Nitrite-oxidizing bacteria (NOB), which perform the second step of aerobic nitrification, play an important role in soil. In the present study, we report a novel isolate from agricultural soil affiliated with the genus Nitrobacter and its physiological characteristics. We sampled the surface soil of a vegetable field and obtained mixed culture A31 using the most probable number (MPN) method with inorganic medium containing 0.75‍ ‍mM urea (pH 5.5). The dilution-extinction procedure on culture A31 led to the isolation of a strain that was designated as Nitrobacter sp. A67. The nxrB1 gene sequence of Nitrobacter sp. A67 (302 bp) was classified into Cluster 5, and the highest sequence identity was 96.10% with Nitrobacter sp. BS5/19. The NO₂^- oxidation activity of Nitrobacter sp. A67 was investigated at various pH. The optimum pH for NO₂^- oxidation was 5.8-6.4. This result indicates that Nitrobacter sp. A67 is a moderately acidophilic nitrite-oxidizing bacterium.

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从供应尿素的硝化细菌群落中分离出中等嗜酸性硝化细菌

亚硝酸盐氧化细菌（NOB）执行好氧硝化的第二步，在土壤中发挥着重要作用。在本研究中，我们报告了从农业土壤中分离出的一种隶属于硝化细菌属的新菌株及其生理特征。我们对一块菜地的表层土壤进行了取样，并采用最可能数（MPN）法，在含有 0.75‍ ‍mM 尿素（pH 5.5）的无机培养基中获得了混合培养物 A31。通过对培养物 A31 的稀释-消减程序，分离出一株菌株，命名为硝化细菌 A67。硝化细菌 A67 的 nxrB1 基因序列（302 bp）被归入第 5 组，与硝化细菌 BS5/19 的最高序列同一性为 96.10%。研究了硝化细菌 A67 在不同 pH 值下的 NO2- 氧化活性。氧化 NO2- 的最佳 pH 值为 5.8-6.4。这一结果表明，硝化细菌 A67 是一种中度嗜酸性亚硝酸盐氧化细菌。

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.