ChangPu YuJin Tang improves Tourette disorder symptoms by modulating amino acid neurotransmitters in IDPN model rats.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Metabolic brain disease Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI:10.1007/s11011-024-01411-x

Man-Qi Lu, Zheng-Gang Shi, Jing Shang, Lü Gao, Lei Gao, Wei-Jiao Gao

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Abstract

Introduction: Changpu Yujin Tang(CPYJT), a Chinese herbal compound, is an effective therapeutic strategy for pediatric patients with Tourette disorder (TD). Therefore, this work aims to investigate the therapeutic mechanisms of CPYJT.

Methods: Behavioral and cellular ultrastructural evaluation of the therapeutic effects of CPYJT in TD model rats. Colorimetric methods, reverse transcription‑quantitative PCR, and Western Blot were used to measure the altered levels of GLU, GABA, and the levels of VGLUT1, GLUD1, GABRA3, and GAD65 in the cortex, striatum, and thalamus of the TD model rats after 7, 14, 21, and 28 days of CPYJT administration.

Results: CPYJT significantly reduced stereotypic behavior and motor behavior scores in TD model rats. CPYJT ameliorates myelin structural damage in TD model rat neuronal cells. CPYJT decreased GLU content, elevated GABA content, decreased GLUD1 and VGLUT1 levels, and elevated GAD65 and GABRA3 levels in TD model rats' cortex, striatum, and thalamus. CPYJT has different regulatory time points in the cortex, striatum, and thalamus for critical factors of amino acid-based neurotransmission.

Conclusion: CPYJT protects behavioral and structural damage of neuronal cells in multiple brain regions in TD model rats.

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常朴郁金汤通过调节氨基酸神经递质改善IDPN模型大鼠的抽动症症状

简介长普玉真汤（CPYJT）是一种中药复方制剂，是治疗小儿抽动症（TD）的有效药物。因此，本研究旨在探讨长普育金汤的治疗机制：方法：通过行为和细胞超微结构评估 CPYJT 对 TD 模型大鼠的治疗效果。采用比色法、逆转录-定量 PCR 和 Western Blot 检测给药 7、14、21 和 28 天后 TD 模型大鼠皮层、纹状体和丘脑中 GLU、GABA 水平的变化以及 VGLUT1、GLUD1、GABRA3 和 GAD65 的水平：结果：CPYJT能明显减少TD模型大鼠的刻板行为和运动行为评分。CPYJT 可改善 TD 模型大鼠神经细胞的髓鞘结构损伤。CPYJT 降低了 TD 模型大鼠大脑皮层、纹状体和丘脑中 GLU 的含量，提高了 GABA 的含量，降低了 GLUD1 和 VGLUT1 的水平，提高了 GAD65 和 GABRA3 的水平。CPYJT在大脑皮层、纹状体和丘脑中对基于氨基酸的神经传递的关键因素具有不同的调节时间点：结论：CPYJT 可保护 TD 模型大鼠多个脑区神经细胞的行为和结构损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolic brain disease 医学-内分泌学与代谢

CiteScore

5.90

自引率

5.60%

发文量

248

审稿时长

6-12 weeks

期刊介绍： Metabolic Brain Disease serves as a forum for the publication of outstanding basic and clinical papers on all metabolic brain disease, including both human and animal studies. The journal publishes papers on the fundamental pathogenesis of these disorders and on related experimental and clinical techniques and methodologies. Metabolic Brain Disease is directed to physicians, neuroscientists, internists, psychiatrists, neurologists, pathologists, and others involved in the research and treatment of a broad range of metabolic brain disorders.