A promising approach for quantifying focal stroke modeling and assessing stroke progression: optical resolution photoacoustic microscopy photothrombosis.

IF 5.9 2区 医学 Q2 CELL BIOLOGY Neural Regeneration Research Pub Date : 2025-07-01 Epub Date: 2024-06-03 DOI:10.4103/NRR.NRR-D-23-01617
Xiao Liang, Xingping Quan, Xiaorui Geng, Yujing Huang, Yonghua Zhao, Lei Xi, Zhen Yuan, Ping Wang, Bin Liu
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Abstract

JOURNAL/nrgr/04.03/01300535-202507000-00025/figure1/v/2024-09-09T124005Z/r/image-tiff To investigate the mechanisms underlying the onset and progression of ischemic stroke, some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex. However, these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied, although ischemic stroke is strongly age-related. In this study, we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser. We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation. Moreover, we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke. Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages, thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.

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量化局灶性中风模型和评估中风进展的有效方法:光学分辨率光声显微镜光血栓形成。
JOURNAL/nrgr/04.03/01300535-202507000-00025/figure1/v/2024-09-09T124005Z/r/image-tiff为了研究缺血性中风的发生和发展机制,人们提出了一些可以同时监测和在动物大脑皮层形成栓塞的方法。然而,这些方法往往需要复杂的系统,而且年龄对脑栓塞的影响尚未得到充分研究,尽管缺血性中风与年龄密切相关。在本研究中,我们提出了一种基于光学分辨光声显微镜的可视化光栓形成方法,利用 532 nm 脉冲激光同时创建和监测小鼠缺血性中风。我们观察了不同年龄小鼠的成型过程,并呈现了与年龄相关的血管栓塞分化。此外,我们还结合光学相干断层血管造影术研究了中风后与年龄相关的脑血管变化趋势。我们的成像数据和定量分析强调了不同年龄的小鼠脑血管对中风的不同反应,从而突出了该技术在评估脑血管健康和揭示缺血性中风的年龄相关机制方面的潜力。
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来源期刊
Neural Regeneration Research
Neural Regeneration Research CELL BIOLOGY-NEUROSCIENCES
CiteScore
8.00
自引率
9.80%
发文量
515
审稿时长
1.0 months
期刊介绍: Neural Regeneration Research (NRR) is the Open Access journal specializing in neural regeneration and indexed by SCI-E and PubMed. The journal is committed to publishing articles on basic pathobiology of injury, repair and protection to the nervous system, while considering preclinical and clinical trials targeted at improving traumatically injuried patients and patients with neurodegenerative diseases.
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