Detection of EPEC and STEC strains isolated from children with diarrhea in Argentina.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-09-13 DOI:10.1016/j.ram.2024.07.003
Daniel Fernández Fellenz, Julia M Ruiz, Analía I Etcheverría, Rocio Colello, María V Velez, Marcelo E Sanz, Mónica D Sparo, Sabina Lissarrague, Josefina Pereyra, Gustavo Zanelli, Nora L Padola
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Abstract

Rectal swabs (122) from pediatric patients were analyzed by polymerase chain reaction (PCR) for the detection of EPEC and STEC. STEC isolates were tested for the presence of stx1, stx2, eae, saa and ehxA. All eae-positive samples were tested for the presence of bfpA, and antigen O was determined using the agglutination test. Int1 and Int2 were detected to identify the presence of integrons class 1 and 2, respectively. Escherichia coli was detected in 68% of the samples, of which 18.8% were STEC (2.45%) and EPEC (16.3%). Serogroups STEC O145 and EPEC O130, O113 and O157 were observed, while three strains were non-typable. None of the EPEC strains carrying tbfpA and class 1 and 2 integrons was detected in any of the samples. The results obtained are important considering the virulence profiles found in the isolated EPEC and STEC strains and the serogroups associated with disease in humans.

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检测从阿根廷腹泻儿童中分离出的 EPEC 和 STEC 菌株。
通过聚合酶链反应 (PCR) 分析了儿科患者的直肠拭子(122 份),以检测 EPEC 和 STEC。对 STEC 分离物进行了 stx1、stx2、eae、saa 和 ehxA 检测。对所有 eae 阳性样本进行了 bfpA 检测,并使用凝集试验确定了抗原 O。检测 Int1 和 Int2 分别是为了确定是否存在 1 类和 2 类整合子。68% 的样本中检测到了大肠杆菌,其中 18.8% 是 STEC(2.45%)和 EPEC(16.3%)。发现了血清型为 STEC O145 和 EPEC O130、O113 和 O157 的菌株,还有三株菌株无法分型。在所有样本中均未检测到携带 tbfpA 和 1、2 类整合子的 EPEC 菌株。考虑到在分离出的 EPEC 和 STEC 菌株中发现的毒力特征以及与人类疾病相关的血清群,所获得的结果非常重要。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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