Comparison of tissue and urine microbiota in male, intervention naive patients with and without non-invasive bladder cancer.

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY Urologia Internationalis Pub Date : 2024-09-21 DOI:10.1159/000541296

Muhammed S Ozer, Canet Incir, Huseyin A Yildiz, Muslim D Deger, Alper E Sarikaya, Yesim Tuncok, Gul Ergor, Nuran Esen, Volkan Sen, Ozan Bozkurt, Adil Esen

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Abstract

BACKGROUNDː To investigate the presence of dysbiosis in patients with naive bladder cancer. METHODSː Twelve male patients with non-invasive bladder cancer and twelve age-matched healthy males had midstream urine and tissue samples taken. A history of endourological interventions was determined as an exclusion criterion, ensuring that the study was designed solely with naïve participants. The bacterial 16s ribosomal RNA V3-V4 regions were used to examine urine and tissue samples. We compared the microbiota composition of the bladder cancer and control groups. RESULTSː Escherichia Shigella (p<0.001), Staphylococcus (p<0.001), Delftia (p<0.001), Acinetobacter (p<0.001), Corynebacterium (p<0.001), and Enhydrobacter (p<0.001) were abundant in bladder cancer tissue samples. Escherichia Shigella (p<0.001), Ureaplasma (p<0.001), Lactobacillus (p=0.005), Stenotrophomonas (p<0.001), Streptococcus (p<0.001), Corynebacterium (p<0.001), and Prevotella (p=0.039) were abundant in bladder cancer urine samples. Midstream urine has a sensitivity of 83% for detecting dysbiotic bacteria in cancer tissue. CONCLUSIONSː Our research is the first microbiota study of bladder cancer done with naive patients who have never had an endourological intervention. Escherichia Shigella, Staphylococcus, Acinetobacter, Enhydrobacter, Delftia, Corynebacterium, and Pseudomonas were detected as dysbiotic bacteria in bladder cancer. The sensitivity of the midstream urine sample in detecting dysbiosis in tissue is %83.

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男性非侵袭性膀胱癌患者和未接受干预的患者组织和尿液微生物群的比较。

背景ː 研究天真膀胱癌患者体内是否存在菌群失调。方法ː 12 名男性非侵袭性膀胱癌患者和 12 名年龄匹配的健康男性采集了中段尿液和组织样本。将有过内科治疗史的患者作为排除标准，以确保该研究只针对未接受治疗的患者。我们使用细菌 16s 核糖体 RNA V3-V4 区域来检测尿液和组织样本。我们比较了膀胱癌组和对照组的微生物群组成。结果ː 在膀胱癌组织样本中，志贺氏埃希氏菌（p<0.001）、葡萄球菌（p<0.001）、Delftia（p<0.001）、Acinetobacter（p<0.001）、Corynebacterium（p<0.001）和 Enhydrobacter（p<0.001）大量存在。在膀胱癌尿液样本中，志贺氏大肠杆菌（p<0.001）、尿解脲原体（p<0.001）、乳酸杆菌（p=0.005）、血单胞菌（p<0.001）、链球菌（p<0.001）、棒状杆菌（p<0.001）和普雷沃特氏菌（p=0.039）大量存在。中段尿液检测癌症组织中菌群失调细菌的灵敏度为 83%。结论ː 我们的研究是首次对膀胱癌微生物群进行研究，研究对象是从未接受过尿道内介入治疗的天真患者。在膀胱癌中检测到的菌群失调细菌包括志贺氏菌、葡萄球菌、醋酸杆菌、恩氢杆菌、Delftia、棒状杆菌和假单胞菌。中段尿液样本检测组织中菌群失调的灵敏度为 83%。

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来源期刊

Urologia Internationalis 医学-泌尿学与肾脏学

CiteScore

3.30

自引率

6.20%

发文量

审稿时长

3-8 weeks

期刊介绍： Concise but fully substantiated international reports of clinically oriented research into science and current management of urogenital disorders form the nucleus of original as well as basic research papers. These are supplemented by up-to-date reviews by international experts on the state-of-the-art of key topics of clinical urological practice. Essential topics receiving regular coverage include the introduction of new techniques and instrumentation as well as the evaluation of new functional tests and diagnostic methods. Special attention is given to advances in surgical techniques and clinical oncology. The regular publication of selected case reports represents the great variation in urological disease and illustrates treatment solutions in singular cases.