CircFLNA facilitates gastric cancer cell proliferation and glycolysis via regulating SOX5 by sponging miR-1200.

IF 1.1 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Arab Journal of Gastroenterology Pub Date : 2024-09-14 DOI:10.1016/j.ajg.2024.07.012
Xinxing Yang, Dongsheng Li, Yuqin Sun, Lisha Yi, Qiuxian Chen, Yadong Lai
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Abstract

Background and study aims: Circular RNAs (circRNAs) are important regulators for gastric cancer (GC) progression. Our study aims to investigate the role and mechanism of circFLNA in GC progression.

Patients and methods: The levels of circFLNA, microRNA (miR)-1200 and SRY-box transcription factor 5 (SOX5) were examined using qRT-PCR. Flow cytometry, cell counting kit 8 assay and EdU assay were performed to measure cell proliferation and apoptosis. Cell glycolysis ability was assessed by examining glucose uptake and lactate produce. RNA interaction was determined using RNA pull-down assay and dual-luciferase reporter assay. Mice xenograft models were constructed to evaluate the regulation of circFLNA knockdown on GC tumor growth.

Results: CircFLNA was upregulated in GC tissues. Functional experiments showed that circFLNA knockdown suppressed GC cell proliferation, inhibited glycolysis, and promoted apoptosis in vitro, as well as reduced GC tumor growth in vivo. CircFLNA sponged miR-1200, and miR-1200 targeted SOX5. MiR-1200 mimic reversed the promotion effect of circFLNA overexpression on GC cell growth and glycolysis, and SOX5 upregulation also abolished the inhibiting effect of miR-1200 mimic on GC cell growth and glycolysis.

Conclusion: Our data suggest that circFLNA might exert oncogenic role in GC development, which promoted GC proliferation and glycolysis through regulating miR-1200/SOX5 axis.

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CircFLNA 通过海绵状 miR-1200 调节 SOX5,促进胃癌细胞增殖和糖酵解。
背景和研究目的:环状RNA(circRNA)是胃癌(GC)进展的重要调控因子。我们的研究旨在探讨 circFLNA 在 GC 进展中的作用和机制:使用 qRT-PCR 检测 circFLNA、microRNA (miR)-1200 和 SRY-box 转录因子 5 (SOX5) 的水平。流式细胞术、细胞计数试剂盒 8 检测法和 EdU 检测法用于测量细胞增殖和凋亡。通过检测葡萄糖摄取量和乳酸产生量评估细胞糖酵解能力。使用 RNA 拉取试验和双荧光素酶报告试验确定 RNA 相互作用。建立小鼠异种移植模型,评估circFLNA敲除对GC肿瘤生长的调控作用:结果:circFLNA在GC组织中上调。功能实验表明,体外敲除circFLNA可抑制GC细胞增殖、抑制糖酵解、促进细胞凋亡,体内敲除circFLNA可减少GC肿瘤的生长。circFLNA能疏导miR-1200,而miR-1200靶向SOX5。MiR-1200模拟物逆转了circFLNA过表达对GC细胞生长和糖酵解的促进作用,而SOX5的上调也消除了miR-1200模拟物对GC细胞生长和糖酵解的抑制作用:我们的数据表明,circFLNA可能在GC发育过程中发挥致癌作用,通过调控miR-1200/SOX5轴促进GC增殖和糖酵解。
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来源期刊
Arab Journal of Gastroenterology
Arab Journal of Gastroenterology Medicine-Gastroenterology
CiteScore
2.70
自引率
0.00%
发文量
52
期刊介绍: Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.
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