Mauro Cozzolino, Yagmur Ergun, Denis A Seli, Sonia Herraiz
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引用次数: 0
Abstract
Intraovarian injection of platelet-rich plasma (PRP) has been recently proposed, with encouraging results to provide an alternative option to patients diagnosed with POR or POI. However, the broad spectrum of PRP effects on the reproductive function and the mechanisms of action in follicular activation, response to stimulation, and embryo quality have not yet been studied. In this study, we first induced poor ovarian reserve (POR) and premature ovarian insufficiency (POI) ovarian phenotypes in CD1 mice undergoing PRP or sham intraovarian injection. PRP administration reduced those alterations induced by chemotherapy in ovarian stroma and follicle morphology in both the POR and POI conditions. After ovarian stimulation, we found that PRP did not modify the MII-oocyte yield. Nevertheless, the amount of obtained 2-cell embryos and fertilization rate were increased, being especially relevant for the POI model. Further in vitro embryo culture led to improved blastocyst formation rates and higher numbers of good quality blastocysts in PRP vs. sham females in both the POR and POI conditions. These positive results of PRP injection were also validated in the C57Bl/6 stain. Altogether, our findings suggest a possible effect on oocyte and embryo quality. This effect is likely due to the increase of local paracrine signaling through the released growth factors in PRP-treated ovaries.
最近,有人提出在卵巢内注射富血小板血浆(PRP),结果令人鼓舞,为被诊断为 POR 或 POI 的患者提供了另一种选择。然而,PRP 对生殖功能的广泛影响以及在卵泡激活、对刺激的反应和胚胎质量方面的作用机制尚未得到研究。在本研究中,我们首先诱导 CD1 小鼠接受 PRP 或假性卵巢内注射,诱导出卵巢储备不良(POR)和卵巢早衰(POI)的卵巢表型。在POR和POI两种情况下,给予PRP都能减少化疗引起的卵巢基质和卵泡形态的改变。卵巢刺激后,我们发现 PRP 并未改变 MII 卵母细胞的产量。不过,获得的 2 细胞胚胎数量和受精率都有所增加,这与 POI 模型尤为相关。与假雌性相比,在 POR 和 POI 条件下,进一步的体外胚胎培养提高了 PRP 的囊胚形成率,并增加了优质囊胚的数量。PRP 注射的这些积极结果在 C57Bl/6 染色体中也得到了验证。总之,我们的研究结果表明,PRP 可能对卵母细胞和胚胎质量有影响。这种影响可能是由于经 PRP 处理的卵巢通过释放的生长因子增加了局部旁分泌信号。