Standard operating procedure for the analysis of major ions in hydrothermal fluids by ion chromatography.

Open research Europe Pub Date : 2024-07-29 eCollection Date: 2023-01-01 DOI:10.12688/openreseurope.15605.2
Monica Correggia, Luciano Di Iorio, Alessia Benedicta Bastianoni, Mustafa Yucel, Angelina Cordone, Donato Giovannelli
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Abstract

This standard operating procedure (SOP) describes an ion chromatography (IC) procedure for the major cations and anions in hydrothermal fluids. Hydrothermal fluids are aqueous solutions with a wide range of temperature, salinity, pH and ion species that can be used by microbial metabolism as electron donors and electron acceptors. Due to the high variability of the environmental physical-chemical parameters in these samples, we have developed this protocol taking into account the special features of the matrices analyzed. An Eco IC Metrohm system equipped with a conductivity detector was used. Calibration curves are linear in the 0.1 to 10 mg/L concentration range for cations Ca 2+, Na +, K +, Mg 2+, NH 4 + and anions Cl -, Br -, NO 3 -, NO 2 -, SO 4 2- , PO 4 3-.

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利用离子色谱法分析热液中主要离子的标准操作程序。
本标准操作程序(SOP)介绍了热液中主要阳离子和阴离子的离子色谱(IC)程序。热液是一种水溶液,具有广泛的温度、盐度、pH 值和离子种类,可被微生物新陈代谢用作电子供体和电子受体。由于这些样品中的环境物理化学参数变化很大,我们在制定本方案时考虑到了所分析基质的特殊性。我们使用了配备电导检测器的 Eco IC Metrohm 系统。在 0.1 至 10 mg/L 浓度范围内,阳离子 Ca 2+、Na +、K +、Mg 2+、NH 4 + 和阴离子 Cl -、Br -、NO 3 -、NO 2 -、SO 4 2 -、HPO 4 2 - 的校准曲线呈线性关系。
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