Improving monitoring of dissolved organic matter from the wastewater treatment plant to the receiving environment: A new high-frequency in situ fluorescence sensor capable of analyzing 29 pairs of Ex/Em wavelengths.

Angélique Goffin, Gilles Varrault, Nadège Musabimana, Antoine Raoult, Metehan Yilmaz, Sabrina Guérin-Rechdaoui, Vincent Rocher
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Abstract

A high-frequency, in situ fluorescence probe, called Fluocopée®, has been developed in order to better monitor variations in both the quality and quantity of dissolved organic matter within various aquatic environments (e.g. wastewater, receiving environments) thanks to a wide choice of 29 measured Excitation/Emission wavelength pairs. This advance pave the way to new measurement possibilities in comparison with existing probes, which are usually only able to measure 1-4 fluorophores. The qualification tests of the Fluocopée® probe indicate a high level of accuracy for the measurements of tyrosine, tryptophan and humic acids solutions. Good repeatability and reproducibility are also observed. For the first time, this tool has been deployed in an urban watershed (Bougival, Seine River, downstream of Paris) and in the settled effluent from a wastewater treatment plant (Seine aval, Achères, France). This new high-frequency in situ probe offers great application potential, including organic matter quality and quantity monitoring at drinking and wastewater treatment plants (treatment optimization) and in continental and marine waters (the fate of organic matter in biogeochemical cycles).

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改进对从污水处理厂到接收环境的溶解有机物的监测:能够分析 29 对 Ex/Em 波长的新型高频原位荧光传感器。
为了更好地监测各种水生环境(如废水、受纳环境)中溶解有机物的质和量的变化,我们开发了一种名为 Fluocopée® 的高频原位荧光探头,该探头有 29 种可测量的激发/发射波长对可供选择。与通常只能测量 1-4 种荧光团的现有探头相比,这一进步为新的测量可能性铺平了道路。Fluocopée® 探头的鉴定测试表明,它在测量酪氨酸、色氨酸和腐殖酸溶液时具有很高的准确性。重复性和再现性也很好。该工具首次应用于城市流域(巴黎塞纳河下游的 Bougival)和污水处理厂的沉淀废水(法国 Achères 的 Seine aval)。这一新的高频原位探测器具有巨大的应用潜力,包括对饮用水和废水处理厂(处理优化)以及大陆和海洋水域(有机物在生物地球化学循环中的归宿)的有机物质量和数量进行监测。
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