{"title":"A DNA Molecular Logic Circuit for Precise Tumor Identification.","authors":"Yingyu Sima, Lili Ai, Linlin Wang, Pengge Zhang, Qiang Zhang, Shanchao Wu, Sitao Xie, Zilong Zhao, Weihong Tan","doi":"10.1021/acs.nanolett.4c02342","DOIUrl":null,"url":null,"abstract":"<p><p>Tumor-associated antigens (TAAs) are not exclusively expressed in cancer cells, inevitably causing the \"on target, off tumor\" effect of molecular recognition tools. To achieve precise recognition of cancer cells, by using protein tyrosine kinase 7 (PTK7) as a model TAA, a DNA molecular logic circuit Aisgc8 was rationally developed by arranging H<sup>+</sup>-binding i-motif, ATP-binding aptamer, and PTK7-targeting aptamer Sgc8c in a DNA sequence. Aisgc8 output the conformation of Sgc8c to recognize PTK7 on cells in a simulated tumor microenvironment characterized by weak acidity and abundant ATP, but not in a simulated physiological environment. Through <i>in vitro</i> and <i>in vivo</i> results, Aisgc8 demonstrated its ability to precisely recognize cancer cells and, as a result, displayed excellent performance in tumor imaging. Thus, our studies produced a simple and efficient strategy to construct DNA logic circuits, opening new possibilities to develop convenient and intelligent precision diagnostics by using DNA logic circuits.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Letters","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acs.nanolett.4c02342","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Tumor-associated antigens (TAAs) are not exclusively expressed in cancer cells, inevitably causing the "on target, off tumor" effect of molecular recognition tools. To achieve precise recognition of cancer cells, by using protein tyrosine kinase 7 (PTK7) as a model TAA, a DNA molecular logic circuit Aisgc8 was rationally developed by arranging H+-binding i-motif, ATP-binding aptamer, and PTK7-targeting aptamer Sgc8c in a DNA sequence. Aisgc8 output the conformation of Sgc8c to recognize PTK7 on cells in a simulated tumor microenvironment characterized by weak acidity and abundant ATP, but not in a simulated physiological environment. Through in vitro and in vivo results, Aisgc8 demonstrated its ability to precisely recognize cancer cells and, as a result, displayed excellent performance in tumor imaging. Thus, our studies produced a simple and efficient strategy to construct DNA logic circuits, opening new possibilities to develop convenient and intelligent precision diagnostics by using DNA logic circuits.
肿瘤相关抗原(TAA)并非只在癌细胞中表达,这不可避免地造成分子识别工具的 "脱靶 "效应。为了实现对癌细胞的精准识别,研究人员以蛋白酪氨酸激酶7(PTK7)为TAA模型,将H+结合i-motif、ATP结合适配体和PTK7靶向适配体Sgc8c排列在DNA序列中,合理地开发了DNA分子逻辑电路Aisgc8。在以弱酸性和丰富 ATP 为特征的模拟肿瘤微环境中,Aisgc8 可输出 Sgc8c 的构象以识别细胞上的 PTK7,但在模拟生理环境中则不能。通过体外和体内实验结果,Aisgc8 展示了其精确识别癌细胞的能力,并因此在肿瘤成像中表现出卓越的性能。因此,我们的研究提出了一种简单高效的 DNA 逻辑电路构建策略,为利用 DNA 逻辑电路开发便捷、智能的精准诊断提供了新的可能性。
期刊介绍:
Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including:
- Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale
- Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies
- Modeling and simulation of synthetic, assembly, and interaction processes
- Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance
- Applications of nanoscale materials in living and environmental systems
Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.