"Protective effects of artichoke extract and Bifidobacterium longum on male infertility in diabetic rats"

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-09-23 DOI:10.1016/j.bbrep.2024.101834

Zahra Ansari , Mohammad Hasan Maleki , Fatemeh Roohy , Zahra Ebrahimi , Mesbah Shams , Pooneh Mokaram , Zahra Zamanzadeh , Zahra Hosseinzadeh , Farhad Koohpeyma , Sanaz Dastghaib

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Abstract

Background

Diabetes is a major global health concern and plays a significant role in male infertility and hormonal abnormalities by altering the tissue structure of spermatogenic tubes and decreasing the number of spermatogonia. This study investigated the effect of artichoke (Cynara scolymus L) hydroalcoholic extract and Bifidobacterium longum probiotic on sexual hormones, oxidative stress, apoptosis pathway, and histopathological changes in testicular tissues of diabetic rats to find an adjuvant therapy to manage the infertility complications of diabetes.

Methods

In this experiment, 96 male-rats were randomly selected from eight groups. Control, Sham (normal saline), DM group (IP injected with 60 mg/kg STZ), Cynara (400 mg/kg hydroalcoholic extract of Cynara scolymus L), BBL (received 1 × 10⁹ CFU/ml/day Bifidobacterium longum), DM + Cynara, DM + BBL, and DM + Cynara + BBL groups. After 48 days of orally gavage, serum level of FBS (fasting blood sugar), Malondi-aldehyde (MDA), Total-Anti-Oxidant Capacity (TAC), FSH (Follicle-stimulating hormone), LH (Luteinizing hormone), Testosterone, Testis mRNA-expressions of Protamin (prm1), BCL2, and Caspase-9 genes, as well as stereological changes were measured.

Results

In comparison to the diabetic group, the hydroalcoholic extract of Cynara scolymus L combined with the probiotic Bifidobacterium longum resulted in a substantial decrease in FBS (p < 0.001) and MDA(p < 0.05) concentrations, and the expression of the Caspase-9 gene (1.33-fold change). In addition, serum levels of TAC, LH, FSH, Testosterone were significantly increased (p < 0.05). mRNA expression of protamine (p = 0.016) and BCL2 (0.72-fold change) were detected. Furthermore, in comparison with diabetic rats, the Cynara scolymus L-and Bifidobacterium longum-treated groups showed a significant increase in the number of sexual lineage cells, total weight, sperm count, motility, normal morphology, volume of the testis, and volume and length of seminiferous tubules (p < 0.05).

Conclusion

The findings demonstrated that Cynara scolymus L extract and Bifidobacterium longum supplement had great therapeutic potential, including antioxidant, anti-apoptotic, anti-diabetic, fertility index improvement, and sex hormone modulators.

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"朝鲜蓟提取物和长双歧杆菌对糖尿病大鼠雄性不育症的保护作用"

背景糖尿病是全球关注的主要健康问题，它通过改变生精管道的组织结构和减少精原细胞的数量，在男性不育和激素异常中起着重要作用。本研究探讨了朝鲜蓟（Cynara scolymus L）水醇提取物和长双歧杆菌益生菌对糖尿病大鼠睾丸组织中性激素、氧化应激、细胞凋亡途径和组织病理学变化的影响，以寻找控制糖尿病不育并发症的辅助疗法。对照组、Sham 组（普通生理盐水）、DM 组（IP 注射 60 mg/kg STZ）、Cynara 组（400 mg/kg Cynara scolymus L 水醇提取物）、BBL 组（每天注射 1 × 109 CFU/ml 长双歧杆菌）、DM + Cynara 组、DM + BBL 组和 DM + Cynara + BBL 组。口服48天后，测定血清中FBS（空腹血糖）、丙二醛（MDA）、总抗氧化能力（TAC）、FSH（卵泡刺激素）、LH（促黄体生成素）、睾酮、睾丸中Protamin（prm1）、BCL2和Caspase-9基因的mRNA表达水平以及立体结构变化。结果与糖尿病组相比，Cynara scolymus L 的水醇提取物与益生菌长双歧杆菌（Bifidobacterium longum）结合使用后，FBS（p < 0.001）和 MDA（p < 0.05）浓度大幅降低，Caspase-9 基因的表达量（变化了 1.33 倍）也大幅降低。此外，血清中 TAC、LH、FSH 和睾酮的水平也明显升高（p < 0.05），检测到原胺（p = 0.016）和 BCL2 的 mRNA 表达（变化了 0.72 倍）。此外，与糖尿病大鼠相比，Cynara scolymus L 和长双歧杆菌处理组的性系细胞数量、总重量、精子数量、活力、正常形态、睾丸体积、曲细精管体积和长度均显著增加（p < 0.05）。研究结果表明，Cynara scolymus L 提取物和长双歧杆菌补充剂具有巨大的治疗潜力，包括抗氧化、抗凋亡、抗糖尿病、改善生育指数和性激素调节剂。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.