Bioactive peptides from food waste: New innovative bio-nanocomplexes to enhance cellular uptake and biological effects

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED Food Chemistry Pub Date : 2024-09-18 DOI:10.1016/j.foodchem.2024.141326

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Abstract

Mastitis is the most important bovine disease, causing dramatic economic losses to the dairy industry, worldwide. This study explores the valorization of whey from cows affected by mastitis, through a novel separation approach. Surface Active Maghemite Nanoparticles (SAMNs) were used as magnetic baits to selectively bind bioactive peptides with potential health benefits. Advanced techniques such as HPLC and LC-MS/MS highlighted SAMN capability of isolating a restricted group of peptides, drastically diverging from the control profile (Solid Phase Extraction, SPE) and characterized by a peculiar acidic residue distribution. Most importantly, both magnetically purified and nano-immobilized peptides (SAMN@peptides) showed protective activity against oxidative stress and inflammation, when tested on Caco-2 cells; with SAMN@peptides being associated with the strongest biological effect. SAMNs exhibited excellent characteristics, they are environmentally sustainable, and their synthesis is cost-effective prompting at a scalable and selective tool for capturing bioactive peptides, with potential applications in functional foods and nutraceuticals.

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从食物垃圾中提取生物活性肽：提高细胞吸收和生物效应的新型创新生物纳米复合物

乳腺炎是最重要的牛病，给全球乳业造成了巨大的经济损失。本研究通过一种新颖的分离方法，探讨了从患有乳腺炎的奶牛身上提取乳清的价值。利用表面活性磁铁矿纳米颗粒（SAMNs）作为磁性诱饵，选择性地结合具有潜在健康益处的生物活性肽。HPLC 和 LC-MS/MS 等先进技术突显了 SAMN 分离受限肽群的能力，与对照组（固相萃取，SPE）的情况大相径庭，并以特殊的酸性残留物分布为特征。最重要的是，在对 Caco-2 细胞进行测试时，磁性纯化肽和纳米固定肽（SAMN@肽）都显示出对氧化应激和炎症的保护活性；SAMN@肽具有最强的生物效应。SAMNs 具有优异的特性，它们在环境上是可持续的，而且其合成具有成本效益，这促使其成为捕获生物活性肽的一种可扩展、可选择性的工具，在功能食品和营养保健品中具有潜在的应用价值。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.