Dual-functional probe-based multi-signal immunosensor platform for tropane alkaloids: Verification and evaluation

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-09-17 DOI:10.1016/j.foodchem.2024.141298

Zile Wang , Ke Han , Zhiyue Feng , Boyan Sun , Shuai Zhang , Sihan Wang , Haiyang Jiang

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Abstract

This study aims to realise rapid detecting of tropane alkaloids (TAs) in food. For this purpose, a broad-spectrum single-chain fragment variable was fused with horseradish peroxidase to create an antibody–enzyme complex (AEC) with antigen recognition and catalytic activity. A multi-signal immunosensor platform based on AEC in the direct competitive reaction mode was constructed using 3,3′,5,5′-tetramethylbenzidine and 10-acetyl-3,7-dihydroxyphenoxazine as substrates. The sensitivity of TAs in the immunosensor platform ranged from 0.25 μg/kg to 7912.46 μg/kg. Honey was selected as a representative food sample, and the limit of detection of TAs in honey ranged from 0.02 μg/kg to 409.11 μg/kg, with a recovery rate of 65.7 %–117.1 % and a coefficient of variation less than 21.4 %. Results showed that the immunosensor platform possesses satisfactory accuracy and precision, which highlights its potential for practical applications and its suitability as an ideal tool for rapid screening of TAs in food.

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基于探针的双功能多信号对位生物碱免疫传感器平台：验证与评估

本研究旨在实现食品中托烷生物碱（TA）的快速检测。为此，将广谱单链片段变量与辣根过氧化物酶融合，形成具有抗原识别和催化活性的抗体-酶复合物（AEC）。以 3,3′,5,5′-四甲基联苯胺和 10-乙酰基-3,7-二羟基吩嗪为底物，在直接竞争反应模式下构建了基于 AEC 的多信号免疫传感器平台。免疫传感器平台对 TAs 的灵敏度范围为 0.25 μg/kg 至 7912.46 μg/kg。以蜂蜜为代表性食品样品，蜂蜜中 TAs 的检出限为 0.02 μg/kg 至 409.11 μg/kg，回收率为 65.7 %-117.1 %，变异系数小于 21.4 %。结果表明，该免疫传感器平台具有令人满意的准确度和精密度，突出了其实际应用的潜力，是快速筛查食品中 TAs 的理想工具。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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