Molecular characterization and immune association of Fas-associated death domain protein (FADD) in Tenebrio molitor

IF 1.2 4区 农林科学 Q3 ENTOMOLOGY Entomological Research Pub Date : 2024-09-24 DOI:10.1111/1748-5967.12765
Keunho Yun, Ho Am Jang, Bharat Bhusan Patnaik, Su Hyeon Hwang, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo
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Abstract

Fas-associated death domain protein (FADD) is an apoptotic adapter molecule that recruits caspases and facilitates the formation of death-inducing signaling complexes in mammals. In lower animals, FADD forms a complex with immune deficiency and death-related ced-3/Nedd2-like caspase to elicit antimicrobial responses. In this study, we characterized the FADD sequence of Tenebrio molitor (TmFADD) using molecular informatics to understand its role in immune surveillance of the host against microorganisms. The 618 bp open reading frame of TmFADD encodes a polypeptide of 204 amino acids. The protein includes two promiscuous domains, the death-effector domain and the death domain, found at the N- and C-termini, respectively. TmFADD is clustered with the coleopteran FADD sequences in the phylogenetic tree. Homology-based structural analysis showed an α-helical model with a QMEAN score of 0.58. The expression of TmFADD mRNA was conspicuous in the larval and early pupal stages. TmFADD mRNA was distributed in the immune tissues of the larva and was found predominately in the gut of the adult. Infection of the host with Candida albicans, Escherichia coli or Staphylococcus aureus increased the expression of TmFADD mRNA, suggesting that these microorganisms trigger the innate immunity of the host. The silencing of TmFADD transcripts resulted in an increased susceptibility of T. molitor larvae to infection with E. coli, but not to infection with C. albicans or S. aureus. Hence, these results indicate that FADD plays a role in the defense of T. molitor and could open avenues for understanding insect immunity.

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褐飞虱中 Fas 相关死亡结构域蛋白 (FADD) 的分子特征和免疫关联性
Fas-associated death domain protein(FADD)是一种凋亡适配器分子,可在哺乳动物体内招募caspases并促进死亡诱导信号复合物的形成。在低等动物中,FADD 与免疫缺陷和死亡相关的 ced-3/Nedd2-like caspase 形成复合物,引起抗微生物反应。在这项研究中,我们利用分子信息学对褐斑天牛的 FADD 序列(TmFADD)进行了表征,以了解其在宿主对微生物的免疫监视中的作用。TmFADD 的 618 bp 开放阅读框编码一个 204 个氨基酸的多肽。该蛋白质包括两个杂乱的结构域,即死亡效应结构域和死亡结构域,分别位于 N 端和 C 端。在系统发生树中,TmFADD 与鞘翅目昆虫的 FADD 序列聚集在一起。基于同源性的结构分析表明,TmFADD是一个α螺旋模型,QMEAN得分为0.58。TmFADD mRNA在幼虫期和蛹早期表达明显。TmFADD mRNA分布在幼虫的免疫组织中,并主要存在于成虫的肠道中。宿主感染白色念珠菌、大肠杆菌或金黄色葡萄球菌会增加 TmFADD mRNA 的表达,这表明这些微生物会触发宿主的先天免疫。TmFADD转录本的沉默导致褐飞虱幼虫对大肠杆菌感染的敏感性增加,而对白僵菌或金黄色葡萄球菌感染的敏感性则没有增加。因此,这些结果表明,FADD 在褐飞虱的防御中发挥着作用,并为了解昆虫的免疫力开辟了途径。
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来源期刊
CiteScore
2.50
自引率
7.70%
发文量
64
期刊介绍: Entomological Research is the successor of the Korean Journal of Entomology. Published by the Entomological Society of Korea (ESK) since 1970, it is the official English language journal of ESK, and publishes original research articles dealing with any aspect of entomology. Papers in any of the following fields will be considered: -systematics- ecology- physiology- biochemistry- pest control- embryology- genetics- cell and molecular biology- medical entomology- apiculture and sericulture. The Journal publishes research papers and invited reviews.
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