Pengpeng Liu, Karthikeyan Ponnienselvan, Thomas Nyalile, Sarah Oikemus, Anya T. Joynt, Sukanya Iyer, Karen Kelly, Dongsheng Guo, Pyae P. Kyawe, Emma Vanderleeden, Sambra D. Redick, Lei Huang, Zexiang Chen, Jeong Min Lee, Celia A. Schiffer, David M. Harlan, Jennifer P. Wang, Charles P. Emerson, Nathan D. Lawson, Jonathan K. Watts, Erik J. Sontheimer, Jeremy Luban, Scot A. Wolfe
{"title":"Increasing intracellular dNTP levels improves prime editing efficiency","authors":"Pengpeng Liu, Karthikeyan Ponnienselvan, Thomas Nyalile, Sarah Oikemus, Anya T. Joynt, Sukanya Iyer, Karen Kelly, Dongsheng Guo, Pyae P. Kyawe, Emma Vanderleeden, Sambra D. Redick, Lei Huang, Zexiang Chen, Jeong Min Lee, Celia A. Schiffer, David M. Harlan, Jennifer P. Wang, Charles P. Emerson, Nathan D. Lawson, Jonathan K. Watts, Erik J. Sontheimer, Jeremy Luban, Scot A. Wolfe","doi":"10.1038/s41587-024-02405-x","DOIUrl":null,"url":null,"abstract":"<p>In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.</p>","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":null,"pages":null},"PeriodicalIF":33.1000,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1038/s41587-024-02405-x","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.
期刊介绍：
Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research.
The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field.
Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology.
In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.
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