Effect of solution environment on the binding properties of ruthenium(II) complexes [Ru(bim)2(7-CH3-dppz)]2+ and [Ru(bim)2(dppx)]2+ with double-stranded RNA poly(A)·poly(U)

IF 2.7 3区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR Inorganica Chimica Acta Pub Date : 2024-09-24 DOI:10.1016/j.ica.2024.122387

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Abstract

Two new ruthenium(Ⅱ) complexes containing the same ancillary ligands and different intercalating ligands, [Ru(bim)₂(7-CH₃-dppz)]²⁺ (Ru1, bim = 2,2′-biimidazole, 7-CH₃-dppz = 7-methyl-dipyrido-[3,2-a,2′,3′-c]-phenazine) and [Ru(bim)₂(dppx)]²⁺ (Ru2, dppx = 7,8-dimethyldipyridophenazine), have been synthesized and characterized in this work, and the interactions of Ru1 and Ru2 with double-stranded RNA poly(A)·poly(U) have been comparatively studied under both dilute and molecular crowding conditions. Analysis of spectral titrations and viscosity experiments as well as thermal denaturation experiments suggests that although complexes Ru1 and Ru2 in both dilute and molecular crowding solutions bind to poly(A)·poly(U) via intercalation, while the binding and stabilizing effects of the two complexes toward poly(A)·poly(U) under molecular crowding conditions significantly decreases in comparision with those in dilute solutions, suggesting that molecular crowding has a significant weakening effect on the interaction of the two complexes with poly(A)-poly(U). The results obtained will contribute to the understanding of the effects of molecular crowding conditions on the binding and stabilization of double-stranded RNA by metal complexes, in particular Ru(II) complexes.

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溶液环境对钌(II)配合物[Ru(bim)2(7-CH3-dppz)]2+和[Ru(bim)2(dppx)]2+与双链 RNA 聚(A)-聚(U)结合特性的影响

含有相同辅助配体和不同插层配体的两种新钌（Ⅱ）配合物 [Ru(bim)2(7-CH3-dppz)]2+ （Ru1、bim = 2,2′-双咪唑，7-CH3-dppz = 7-甲基-二吡啶-[3,2-a,2′,3′-c]-吩嗪）和 [Ru(bim)2(dppx)]2+ （Ru2、dppx = 7,8-二甲基二哒嗪），并比较研究了 Ru1 和 Ru2 在稀释和分子拥挤条件下与双链 RNA 聚（A）-聚（U）的相互作用。光谱滴定和粘度实验以及热变性实验的分析表明，虽然 Ru1 和 Ru2 复合物在稀溶液和分子挤入溶液中都是通过插层作用与聚(A)-聚(U)结合的，但这两种复合物在分子挤入条件下对聚(A)-聚(U)的结合和稳定作用比在稀溶液中明显降低，表明分子挤入对这两种复合物与聚(A)-聚(U)的相互作用有明显的削弱作用。研究结果将有助于理解分子拥挤条件对金属配合物（尤其是 Ru(II) 配合物）结合和稳定双链 RNA 的影响。

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来源期刊

Inorganica Chimica Acta 化学-无机化学与核化学

CiteScore

6.00

自引率

3.60%

发文量

440

审稿时长

35 days

期刊介绍： Inorganica Chimica Acta is an established international forum for all aspects of advanced Inorganic Chemistry. Original papers of high scientific level and interest are published in the form of Articles and Reviews. Topics covered include: • chemistry of the main group elements and the d- and f-block metals, including the synthesis, characterization and reactivity of coordination, organometallic, biomimetic, supramolecular coordination compounds, including associated computational studies; • synthesis, physico-chemical properties, applications of molecule-based nano-scaled clusters and nanomaterials designed using the principles of coordination chemistry, as well as coordination polymers (CPs), metal-organic frameworks (MOFs), metal-organic polyhedra (MPOs); • reaction mechanisms and physico-chemical investigations computational studies of metalloenzymes and their models; • applications of inorganic compounds, metallodrugs and molecule-based materials. Papers composed primarily of structural reports will typically not be considered for publication.