Biochemical indices of patients with enteric fever and pancreatitis: A comparative cross-sectional study

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY Practical Laboratory Medicine Pub Date : 2024-09-18 DOI:10.1016/j.plabm.2024.e00429

Nathaniel Ebo Aidoo , Emmanuel Kwaku Ofori , Vincent Boima , Eric Nana Yaw Nyarko , John Cletus Osei , Clement G. Darkwah , Morris O. Gayflor , Seth K. Amponsah , Henry Asare-Anane

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Abstract

Objective

Enteric fever (EF), a potentially fatal febrile illness, is prevalent in developing countries. Elevated levels of lipase and amylase in serum, typically associated with acute pancreatitis (AP), have been observed in patients with EF. The elevated enzymes in both conditions may lead to diagnostic confusion and care delays. This study aimed to determine biochemical indices that are peculiar to EF and AP.

Methods

A cross-sectional comparative study was conducted at the Korle-Bu Teaching Hospital, Ghana. Volunteers were categorized into three groups: EF (n = 32), AP (n = 30) and healthy controls (n = 31). A standard questionnaire was used to collect socio-demographic and clinical information from the participants. Blood and stool samples were obtained, followed by biochemical analysis: total amylase, lipase, pancreatic amylase, serum elastase 1, hepatic enzymes, calcium, magnesium, phosphate, stool colour, stool pH, and stool fat presence.

Results

The AP group displayed higher total amylase, lipase, elastase-1, alkaline phosphatase, aspartate aminotransferase, and gamma-glutamyl transferase levels compared to the EF and control groups (p < 0.05 respectively). Elastase 1 levels were found to be high in all AP participants, whereas no elevations were observed in the EF group. Positive associations were observed in the AP and EF groups for lipase vs total amylase (ρ = .543, p = 0.001; ρ = .543, p = 0.001 for both).

Conclusions

Elevated levels of total/pancreatic amylase and lipase were found to be indicative of a patient with AP and EF. Further, elastase-1 was found to be a good biomarker to distinguish between AP and EF.

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肠炎和胰腺炎患者的生化指标：横断面比较研究

目的肠胃热（EF）是一种可能致命的发热性疾病，在发展中国家很普遍。在肠炎患者中观察到血清中脂肪酶和淀粉酶水平升高，这通常与急性胰腺炎（AP）有关。这两种情况下的酶升高都可能导致诊断混乱和护理延误。本研究旨在确定 EF 和 AP 所特有的生化指标。志愿者被分为三组：EF 组（32 人）、AP 组（30 人）和健康对照组（31 人）。采用标准问卷收集参与者的社会人口学和临床信息。采集血液和粪便样本，然后进行生化分析：总淀粉酶、脂肪酶、胰淀粉酶、血清弹性蛋白酶 1、肝酶、钙、镁、磷酸盐、粪便颜色、粪便 pH 值和粪便脂肪含量。所有 AP 参与者的弹性蛋白酶 1 水平都很高，而 EF 组则没有发现任何升高。在 AP 组和 EF 组中，脂肪酶与总淀粉酶呈正相关（ρ = .543，p = 0.001；两者均为ρ = .543，p = 0.001）。此外，还发现弹性蛋白酶-1是区分 AP 和 EF 的良好生物标志物。

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.