Antonio Del Rio Flores, Rui Zhai, David W. Kastner, Kaushik Seshadri, Siyue Yang, Kyle De Matias, Yuanbo Shen, Wenlong Cai, Maanasa Narayanamoorthy, Nicholas B. Do, Zhaoqiang Xue, Dunya Al Marzooqi, Heather J. Kulik, Wenjun Zhang
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引用次数: 0
Abstract
Azides are energy-rich compounds with diverse representation in a broad range of scientific disciplines, including material science, synthetic chemistry, pharmaceutical science and chemical biology. Despite ubiquitous usage of the azido group, the underlying biosynthetic pathways for its formation remain largely unknown. Here we report the characterization of an enzymatic route for de novo azide construction. We demonstrate that Tri17, a promiscuous ATP- and nitrite-dependent enzyme, catalyses organic azide synthesis through sequential N-nitrosation and dehydration of aryl hydrazines. Through biochemical, structural and computational analyses, we further propose a plausible molecular mechanism for azide synthesis that sets the stage for future biocatalytic applications and biosynthetic pathway engineering.
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Nature Chemistry is a monthly journal that publishes groundbreaking and significant research in all areas of chemistry. It covers traditional subjects such as analytical, inorganic, organic, and physical chemistry, as well as a wide range of other topics including catalysis, computational and theoretical chemistry, and environmental chemistry.
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