Does Intralesional Steroid Injection Effectively Mitigate Vocal Fold Scarring in A Rabbit Model?

IF 2.2 3区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Laryngoscope Pub Date : 2024-10-01 DOI:10.1002/lary.31782

Jun-Yeong Jeong, Samjhana Thapa, Seung-Won Lee

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Abstract

Objectives: To assess the efficacy of intralesional steroid treatment in preventing vocal fold scarring following vocal fold surgery using a rabbit model.

Methods: The research involved 42 male New Zealand white rabbits. Fourteen rabbits underwent vocal fold scar surgery using a 532nm laser and served as controls (control group). The remaining rabbits were divided into two groups of 14: one group received vocal fold scar surgery followed by dexamethasone injection (Dexa group) and the other received the same surgery followed by triamcinolone injection (Triam group). Four weeks after surgery, histological examinations and high-speed video analyses of vocal fold vibration were conducted. The maximum amplitude of vibration was the primary measure for assessing vocal fold function. In addition, real-time polymerase chain reaction (PCR) studies were undertaken to analyze scar regeneration and remodeling.

Results: The maximum amplitude differences were notably higher in the Dexa and Triam groups than in controls. Histologically, the collagen density (CD) ratios in both the Dexa and Triam groups were significantly reduced compared with controls. Real-time PCR analysis indicated marked elevations of Has-2 and Mmp-9 in the Dexa and Triam groups relative to controls.

Conclusions: Intralesional steroid injections after vocal fold surgery are effective for reducing vocal fold scarring in a rabbit model.

Level of evidence: NA Laryngoscope, 2024.

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局部注射类固醇是否能有效缓解兔模型声带褶皱瘢痕？

目的以兔子为模型，评估声带手术后局部类固醇治疗对预防声带瘢痕形成的效果：研究涉及 42 只雄性新西兰白兔。其中 14 只兔子接受了 532nm 激光声带瘢痕手术，作为对照组（对照组）。其余 14 只兔子分为两组：一组接受声带瘢痕手术后注射地塞米松（Dexa 组），另一组接受同样的手术后注射曲安奈德（Triam 组）。手术四周后，对声带振动进行组织学检查和高速视频分析。振动的最大振幅是评估声带功能的主要指标。此外，还进行了实时聚合酶链反应（PCR）研究，以分析疤痕再生和重塑情况：结果：Dexa 和 Triam 组的最大振幅差异明显高于对照组。从组织学角度看，与对照组相比，Dexa 组和 Triam 组的胶原蛋白密度 (CD) 比值明显降低。实时 PCR 分析表明，与对照组相比，Dexa 组和 Triam 组的 Has-2 和 Mmp-9 明显升高：结论：在兔子模型中，声带手术后局部注射类固醇可有效减少声带瘢痕：NA 《喉镜》，2024年。

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来源期刊

Laryngoscope 医学-耳鼻喉科学

CiteScore

6.50

自引率

7.70%

发文量

500

审稿时长

2-4 weeks

期刊介绍： The Laryngoscope has been the leading source of information on advances in the diagnosis and treatment of head and neck disorders since 1890. The Laryngoscope is the first choice among otolaryngologists for publication of their important findings and techniques. Each monthly issue of The Laryngoscope features peer-reviewed medical, clinical, and research contributions in general otolaryngology, allergy/rhinology, otology/neurotology, laryngology/bronchoesophagology, head and neck surgery, sleep medicine, pediatric otolaryngology, facial plastics and reconstructive surgery, oncology, and communicative disorders. Contributions include papers and posters presented at the Annual and Section Meetings of the Triological Society, as well as independent papers, "How I Do It", "Triological Best Practice" articles, and contemporary reviews. Theses authored by the Triological Society’s new Fellows as well as papers presented at meetings of the American Laryngological Association are published in The Laryngoscope. • Broncho-esophagology • Communicative disorders • Head and neck surgery • Plastic and reconstructive facial surgery • Oncology • Speech and hearing defects