Double-stranded DNA antibody test using BioPlex220 system: unacceptable option for SLE diagnosis and follow-up.

Kamran Kadkhoda, Gordon Schroeder, Nicole Boyert, Matthew Dee
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Abstract

The anti-double-stranded (ds)DNA antibody test is an integral part of diagnosing systemic lupus erythematosus when the entry criterion is satisfied. We investigated the sensitivity of the BioPlex 2200 instrument compared with the serological gold standard and other tests and clinical information. The results showed an unacceptable sensitivity for this method. Laboratories should be cognizant of this shortcoming when selecting this platform for dsDNA antibody testing.

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使用 BioPlex220 系统进行双链 DNA 抗体检测:诊断和随访系统性红斑狼疮的不可接受的选择。
抗双链(ds)DNA 抗体检测是诊断符合入选标准的系统性红斑狼疮不可或缺的一部分。我们将 BioPlex 2200 仪器的灵敏度与血清学金标准、其他检测方法和临床信息进行了比较。结果显示,该方法的灵敏度令人无法接受。实验室在选择该平台进行dsDNA抗体检测时应认识到这一缺陷。
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