Multifunctional near-infrared fluorescent probe for sensing of lysine and Cu²⁺/Fe³⁺ and relay detection of biothiols.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-25 DOI:10.1016/j.talanta.2024.126944

Yu Shi, Jirui Yu, Yanxi Song, Ji Fan, Xiwen Wang, Shiji Li, Hongqi Li

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Abstract

Lysine (Lys), Cu²⁺ and Fe³⁺ ions and biothiols are essential to a myriad of biological and pathological pathways, and their dysregulation is implicated in a variety of diseases. Development of fluorescent probes capable of detecting multiple analytes may be of great significance for early and accurate diagnosis of diseases and remains a huge challenge. In this context, a novel coumarin-dicyanoisophorone-based probe, engineered for the concurrent sensing of Lys, Cu²⁺, Fe³⁺ and biothiols was developed. The probe exhibited turn-on response to Lys, colorimetric and turn-off response to Cu²⁺ by formation of the probe-Cu²⁺ complex, and ratiometric sensing of Fe³⁺. In addition, the probe-Cu²⁺ complex served colorimetric and fluorescence turn-on sensor for biothiols. The limit of detection (LOD) values for the analytes were in the range of 0.30-4.40 μM. Sensing mechanisms based on intramolecular charge transfer (ICT) and iron-mediated hydrolysis of Schiff base were proposed and substantiated through density functional theory (DFT) calculations. Application of the probe for living cell bioimaging was demonstrated.

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感应赖氨酸和 Cu2+/Fe3+ 以及中继检测生物硫醇的多功能近红外荧光探针。

赖氨酸（Lys）、Cu2+ 和 Fe3+ 离子以及生物硫醇对无数生物和病理途径至关重要，它们的失调与多种疾病有关。开发能够检测多种分析物的荧光探针可能对疾病的早期准确诊断具有重要意义，但这仍然是一个巨大的挑战。在此背景下，我们开发了一种基于香豆素-二氰异佛尔酮的新型探针，可同时感知 Lys、Cu2+、Fe3+ 和生物硫醇。该探针对 Lys 具有开启响应，通过形成探针-Cu2+ 复合物对 Cu2+ 具有比色和关闭响应，对 Fe3+ 具有比率感应。此外，探针-Cu2+ 复合物还是生物硫醇的比色和荧光开启传感器。分析物的检测限（LOD）值范围为 0.30-4.40 μM。通过密度泛函理论（DFT）计算，提出并证实了基于分子内电荷转移（ICT）和铁介导的席夫碱水解的传感机制。研究还展示了该探针在活细胞生物成像中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.