TIM-3-driven macrophage polarisation is associated to recalcitrant chronic rhinosinusitis with nasal polyps.

IF 2.1 4区医学 Q2 OTORHINOLARYNGOLOGY Acta Otorhinolaryngologica Italica Pub Date : 2024-08-01 DOI:10.14639/0392-100X-N2717

Tao Jiang, Tao Yu, Lu Jiang, Zongjing Tong

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Abstract

Objective: This study evaluated the expression of TIM-3 and its influence on macrophage polarisation in recalcitrant chronic rhinosinusitis with nasal polyps (CRSwNP).

Methods: We detected TIM-3 expression in serum and tissue samples of healthy controls (HC), primary CRSwNP, and patients with recurrent CRSwNP. Macrophage markers were detected among three groups, and their correlations with TIM-3 levels were examined. Macrophages from circulating blood were collected and used to examine the impact of TIM-3 on polarisation in vitro.

Results: TIM-3 levels were enhanced in the CRSwNP group compared to the HC group. Tissue immunofluorescence revealed elevated TIM-3 expression in patients with CRSwNP, and patients with multiple recurrences exhibited higher TIM-3 levels compared to their first recurrence and baseline levels. Tissue CD163 and CD206 levels were higher in recurrent CRSwNP in comparison with primary cases and HCs, and had a positive correlation with TIM-3 levels. TIM-3 overexpression promoted M2 polarisation and enhanced TGF-β1 and IL-10 secretion.

Conclusions: TIM-3 expression was enhanced in patients with CRSwNP, especially in those undergoing revision surgeries. TIM-3 may be a novel biomarker for recalcitrant CRSwNP. TIM-3-driven M2 polarisation might be involved in the mechanisms of recurrent CRSwNP.

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TIM-3 驱动的巨噬细胞极化与伴有鼻息肉的顽固性慢性鼻炎有关。

目的：本研究评估了TIM-3的表达及其对慢性鼻炎伴鼻息肉（CRSwNP）患者巨噬细胞极化的影响：本研究评估了TIM-3的表达及其对复发性慢性鼻炎伴鼻息肉（CRSwNP）患者巨噬细胞极化的影响：我们检测了健康对照组（HC）、原发性 CRSwNP 和复发性 CRSwNP 患者血清和组织样本中 TIM-3 的表达。检测了三组患者的巨噬细胞标记物，并研究了它们与 TIM-3 水平的相关性。收集循环血液中的巨噬细胞，用于研究 TIM-3 对体外极化的影响：结果：与 HC 组相比，CRSwNP 组的 TIM-3 水平有所提高。组织免疫荧光显示，CRSwNP 患者的 TIM-3 表达升高，与首次复发和基线水平相比，多次复发患者的 TIM-3 水平更高。与原发病例和 HCs 相比，复发性 CRSwNP 患者的组织 CD163 和 CD206 水平更高，且与 TIM-3 水平呈正相关。TIM-3的过表达促进了M2极化，并增强了TGF-β1和IL-10的分泌：结论：TIM-3在CRSwNP患者中表达增强，尤其是在接受翻修手术的患者中。TIM-3可能是顽固性CRSwNP的新型生物标志物。TIM-3驱动的M2极化可能与复发性CRSwNP的机制有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Otorhinolaryngologica Italica OTORHINOLARYNGOLOGY-

CiteScore

3.40

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acta Otorhinolaryngologica Italica first appeared as “Annali di Laringologia Otologia e Faringologia” and was founded in 1901 by Giulio Masini. It is the official publication of the Italian Hospital Otology Association (A.O.O.I.) and, since 1976, also of the Società Italiana di Otorinolaringoiatria e Chirurgia Cervico-Facciale (S.I.O.Ch.C.-F.). The journal publishes original articles (clinical trials, cohort studies, case-control studies, cross-sectional surveys, and diagnostic test assessments) of interest in the field of otorhinolaryngology as well as clinical techniques and technology (a short report of unique or original methods for surgical techniques, medical management or new devices or technology), editorials (including editorial guests – special contribution) and letters to the Editor-in-Chief. Articles concerning science investigations and well prepared systematic reviews (including meta-analyses) on themes related to basic science, clinical otorhinolaryngology and head and neck surgery have high priority.