Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca²⁺-CaM signaling pathway.

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL Clinics Pub Date : 2024-09-23 eCollection Date: 2024-01-01 DOI:10.1016/j.clinsp.2024.100485

Yang Song, Yang Wang, Ming Li, Yujuan Wang, Tianshu Xu

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Abstract

Objective: In the context of postoperative anal pain, understanding the intricate mechanisms and effective interventions is paramount. This study investigates the role of Muscarinic Acetylcholine Receptors (mAChRs) and the IP3-Ca²⁺-CaM signaling pathway in a rat model of postoperative anal pain, exploring the potential analgesic effects of electroacupuncture.

Methods: Comprehensive approaches involving mechanical sensitivity assays, Western blotting, immunohistochemistry, and intracellular calcium concentration measurement were used.

Results: The authors found elevated mAChRs expression in the postoperative pain model. Antagonizing mAChRs reduced pain sensitivity and attenuated the IP3-Ca²⁺-CaM pathway. Remarkably, electroacupuncture treatment further mitigated pain, potentially by suppressing this signaling cascade.

Interpretation: These findings reveal a novel connection between mAChRs and the IP3-Ca²⁺-CaM pathway in postoperative anal pain and suggest electroacupuncture as a promising avenue for pain relief through these mechanisms, offering insights into innovative strategies for postoperative pain management.

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电针辰砂穴治疗痔疮切除术后肛门疼痛的机制探讨mAChRs/IP3-Ca2+-CaM信号通路的启示。

目的：对于术后肛门疼痛，了解其复杂的机制和有效的干预措施至关重要。本研究探讨了 Muscarinic 乙酰胆碱受体（mAChRs）和 IP3-Ca2+-CaM 信号通路在大鼠术后肛门疼痛模型中的作用，并探索了电针的潜在镇痛效果：方法：采用机械敏感性测定、Western印迹、免疫组织化学和细胞内钙浓度测量等综合方法：结果：作者发现术后疼痛模型中 mAChRs 表达升高。拮抗 mAChRs 可降低疼痛敏感性并减弱 IP3-Ca2+-CaM 通路。值得注意的是，电针治疗进一步减轻了疼痛，这可能是通过抑制这一信号级联实现的：这些研究结果揭示了术后肛门疼痛中 mAChRs 与 IP3-Ca2+-CaM 通路之间的新联系，并表明电针是通过这些机制缓解疼痛的一种很有前景的途径，从而为术后疼痛管理的创新策略提供了启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.