Anti-Mullerian Hormone Induces Foxo1 and Sirt1 Genes Expression in Mouse Ovary.

IF 2.2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Current pharmaceutical biotechnology Pub Date : 2024-09-26 DOI:10.2174/0113892010293250240917143811

Hoda M Abouzeid, Ingy M Hashad, Mona Rady, Sahar M Abdel-Maksoud

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Abstract

Background: Anti-Mullerian hormone (AMH) plays a pivotal role in follicular growth and atresia. Recent studies highlighted the role of AMH in attenuating granulosa cell apoptosis and subsequent follicular atresia. Despite the raising understanding of the role of AMH in folliculogenesis, and its contribution to the pathophysiology of certain diseases such as polycystic ovary syndrome, the effect of AMH on the expression of genes regulating folliculogenesis is stills limited.

Objective: This study aims to gain insights into the effect of AMH on atresia regulating genes.

Method: In vivo study was performed on C57BL/6J mice injected with AMH for one month. Thereafter, relative gene expression quantification of Foxo1, Sirt1, p53, Bim, and Bax genes were performed using RT-PCR.

Results: In this study, AMH significantly enhanced the expression of Foxo1 and Sirt1 gene compared to the control group. On the contrary, AMH did not modulate the expression of p53, Bim, or Bax genes. AMH was also found to increase serum FSH and LH levels in a dosedependent manner.

Conclusion: This study demonstrated the capability of AMH to induce Foxo1 and Sirt1 genes. Moreover, our study revealed the role of AMH in elevating LH serum level which is a main contributor to the pathophysiology of polycystic ovary syndrome, opening new avenues for the study of AMH as a main contributor to the stalled follicular atresia and growth associated with the disease.

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抗沙勒氏激素诱导小鼠卵巢中 Foxo1 和 Sirt1 基因的表达

背景：抗苗勒氏管激素（AMH）在卵泡生长和闭锁中起着关键作用。最近的研究强调了AMH在减少颗粒细胞凋亡和随后的卵泡闭锁中的作用。尽管人们对AMH在卵泡生成中的作用及其对某些疾病（如多囊卵巢综合征）的病理生理学的贡献有了更多的了解，但AMH对卵泡生成调控基因表达的影响仍然有限：本研究旨在了解AMH对闭锁调节基因的影响：方法：对注射AMH一个月的C57BL/6J小鼠进行体内研究。方法：在 C57BL/6J 小鼠体内注射 AMH 一个月，然后用 RT-PCR 对 Foxo1、Sirt1、p53、Bim 和 Bax 基因进行相对基因表达定量：结果：与对照组相比，AMH能明显提高Foxo1和Sirt1基因的表达。相反，AMH对p53、Bim和Bax基因的表达没有影响。AMH还能以剂量依赖的方式提高血清FSH和LH水平：本研究证明了 AMH 诱导 Foxo1 和 Sirt1 基因的能力。此外，我们的研究还揭示了AMH在提高LH血清水平中的作用，而LH是多囊卵巢综合征病理生理学的一个主要因素，这为研究AMH作为与多囊卵巢综合征相关的卵泡闭锁和生长停滞的主要因素开辟了新途径。

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来源期刊

Current pharmaceutical biotechnology 医学-生化与分子生物学

CiteScore

5.60

自引率

3.60%

发文量

203

审稿时长

6 months

期刊介绍： Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include: DNA/protein engineering and processing Synthetic biotechnology Omics (genomics, proteomics, metabolomics and systems biology) Therapeutic biotechnology (gene therapy, peptide inhibitors, enzymes) Drug delivery and targeting Nanobiotechnology Molecular pharmaceutics and molecular pharmacology Analytical biotechnology (biosensing, advanced technology for detection of bioanalytes) Pharmacokinetics and pharmacodynamics Applied Microbiology Bioinformatics (computational biopharmaceutics and modeling) Environmental biotechnology Regenerative medicine (stem cells, tissue engineering and biomaterials) Translational immunology (cell therapies, antibody engineering, xenotransplantation) Industrial bioprocesses for drug production and development Biosafety Biotech ethics Special Issues devoted to crucial topics, providing the latest comprehensive information on cutting-edge areas of research and technological advances, are welcome. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.